Skip Navigation

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (11)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Flores-Sánchez, I. J.
Right arrow Articles by Ramos-Valdivia, A. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Flores-Sánchez, I. J.
Right arrow Articles by Ramos-Valdivia, A. C.
Agricola
Right arrow Articles by Flores-Sánchez, I. J.
Right arrow Articles by Ramos-Valdivia, A. C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 2002, Vol. 43, No. 12 1502-1509
© 2002 Oxford University Press

Biosynthesis of Sterols and Triterpenes in Cell Suspension Cultures of Uncaria tomentosa

Isvett J. Flores-Sánchez, Jaime Ortega-López, María del Carmen Montes-Horcasitas and Ana C. Ramos-Valdivia1

Departamento de Biotecnología y Bioingeniería. Centro de Investigación y Estudios Avanzados IPN (CINVESTAV-IPN). Av. Politécnico 2508. CP.07360. México, D.F. México

Pectin administered to Uncaria tomentosa cell suspension cultures, was found to increase the production of triterpene acids (ursolic and oleanolic acid), however, neither growth nor sterol accumulation were affected. Cell cultures showed that pectin treatment caused a rapid threefold increase in the activities of enzymes involved in the biosynthesis of C5 and C30 isoprenoid, such as isopentenyl diphosphate isomerase and squalene synthase. The activity of a farnesyl diphosphatase, which could divert the flux of farnesyl diphosphate to farnesol, was two times lower in elicited than in control cells. Elicited cells also transformed more rapidly a higher percentage of [5-3H]mevalonic acid into triterpene acids. Interestingly, addition of terbinafine, an inhibitor of squalene epoxidase, to elicited cell cultures inhibited sterol accumulation while triterpene production was not inhibited. These results suggest that in U. tomentosa cells, both the previously mentioned enzymes and those involved in squalene 2,3-oxide formation play an important regulatory role in the biosynthesis of sterols and triterpenes.

1 Corresponding author: E-mail, aramos@mail.cinvestav.mx; Fax, +52-55-57473800 ext 4305.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Cell PhysiolHome page
M.-H. Lee, J.-H. Jeong, J.-W. Seo, C.-G. Shin, Y.-S. Kim, J.-G. In, D.-C. Yang, J.-S. Yi, and Y.-E. Choi
Enhanced Triterpene and Phytosterol Biosynthesis in Panax ginseng Overexpressing Squalene Synthase Gene
Plant Cell Physiol., August 15, 2004; 45(8): 976 - 984.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.