Skip Navigation

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (18)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Nakanomyo, I.
Right arrow Articles by Fukuda, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nakanomyo, I.
Right arrow Articles by Fukuda, H.
Agricola
Right arrow Articles by Nakanomyo, I.
Right arrow Articles by Fukuda, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 2002, Vol. 43, No. 12 1484-1492
© 2002 Oxford University Press

Preferential and Asymmetrical Accumulation of a Rac Small GTPase mRNA in Differentiating Xylem Cells of Zinnia elegans

Ikuko Nakanomyo1, Benedikt Kost2, Nam-Hai Chua2 and Hiroo Fukuda1

1 Department of Biological Sciences, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033 Japan
2 Laboratory of Plant Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10021-6399, U.S.A.

Rac-type small GTPases are known to function in some cellular processes in plants. To further understand the involvement of Rac type GTPases in plant development, we isolated from cultured Zinnia cells a gene (ZeRAC2) encoding a new Rac-type small GTPase. ZeRAC2 mRNA accumulates preferentially in xylogenic culture and transiently at the time when visible tracheary elements appear. Experiments with ZeRAC2 recombinant proteins demonstrated that ZeRAC2 binds to and hydrolyzes GTP. A GFP–ZeRAC2 fusion protein was localized to the plasma membrane. Together with the fact that ZeRAC2 possesses a putative geranylgeranylation site at the C-terminus, this suggests that ZeRAC2 acts on the plasma membrane. In situ hybridization indicated that ZeRAC2 mRNA accumulates preferentially in xylem parenchyma and tracheary element precursor cells, and surprisingly the accumulation is restricted to the site facing developing tracheary elements.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant CellHome page
H. L. Wong, R. Pinontoan, K. Hayashi, R. Tabata, T. Yaeno, K. Hasegawa, C. Kojima, H. Yoshioka, K. Iba, T. Kawasaki, et al.
Regulation of Rice NADPH Oxidase by Binding of Rac GTPase to Its N-Terminal Extension
PLANT CELL, December 1, 2007; 19(12): 4022 - 4034.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Sagi and R. Fluhr
Production of Reactive Oxygen Species by Plant NADPH Oxidases
Plant Physiology, June 1, 2006; 141(2): 336 - 340.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. Gapper and L. Dolan
Control of Plant Development by Reactive Oxygen Species
Plant Physiology, June 1, 2006; 141(2): 341 - 345.
[Full Text] [PDF]

Home page
 Plant CellHome page
Y. Gu, S. Li, E. M. Lord, and Z. Yang
Members of a Novel Class of Arabidopsis Rho Guanine Nucleotide Exchange Factors Control Rho GTPase-Dependent Polar Growth
PLANT CELL, February 1, 2006; 18(2): 366 - 381.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
T. Brembu, P. Winge, and A. M. Bones
The small GTPase AtRAC2/ROP7 is specifically expressed during late stages of xylem differentiation in Arabidopsis
J. Exp. Bot., September 1, 2005; 56(419): 2465 - 2476.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
K. Ohashi-Ito and H. Fukuda
HD-Zip III Homeobox Genes that Include a Novel Member, ZeHB-13 (Zinnia)/ATHB-15 (Arabidopsis), are Involved in Procambium and Xylem Cell Differentiation
Plant Cell Physiol., December 15, 2003; 44(12): 1350 - 1358.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.