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Plant and Cell Physiology, 2002, Vol. 43, No. 11 1276-1284
© 2002 Oxford University Press

Identification of a Mitochondrial Nucleoside Diphosphate Kinase from the Green Alga Dunaliella tertiolecta

Marinela Ioana Anderca1, Takuya Furuichi1, Reinhard Pinontoan2,3 and Shoshi Muto1,2,4

1 Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa-ku, Nagoya, 464-8601 Japan
2 Nagoya University Bioscience Center, Chikusa-ku, Nagoya, 464-8601 Japan

We isolated a full-length cDNA encoding a nucleoside diphosphate (NDP) kinase from a Dunaliella tertiolecta cDNA library by homology cloning and rapid amplification of cDNA ends-PCR. The cDNA sequence, consisting of 840 bp, contained an open reading frame coding for a 221-amino acid protein. The predicted 24-kDa protein was named DtNDK1. It possesses all the residues involved in nucleotide binding and catalysis and, in its long N-terminus, contains putative mitochondrial targeting peptides. The full-length pre-protein expressed in Escherichia coli as a recombinant N-terminally His-tagged protein was retained in inclusion bodies, totally devoid of NDP kinase activity. Upon expression in yeast cells, the full-length protein His-tagged at the C-terminus was found processed in a soluble form that was lacking the first 67 amino acids from the N-terminus. The mature protein, which was purified by affinity chromatography to near homogeneity, showed NDP kinase activity. Confocal microscopy on yeast cells expressing the recombinant protein revealed the specific mitochondrial localization of DtNDK1 labeled at the C-terminus with green fluorescent protein.

3 Present address: Laboratory of Plant Molecular Genetics, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara, 630-0077 Japan.

4 Corresponding author: E-mail, h44787a@nucc.cc.nagoya-u.ac.jp; Fax, +81-52-789-5206.


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