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Plant and Cell Physiology, 2002, Vol. 43, No. 10 1254-1258
© 2002 Oxford University Press

Short Communications

Zinc Ions Inhibit the Protein–DNA Complex Formation between Cyanobacterial Transcription Factor SmtB and its Recognition DNA Sequences

Eugene Hayato Morita1,2,3,7, Miki Wakamatsu4, Koichi Uegaki5, Noboru Yumoto5, Yoshimasa Kyogoku6 and Hidenori Hayashi1,2

1 Department of Chemistry, Faculty of Science, Ehime University, 2-5 Bunkyo-cho, Matsuyama, Ehime, 790-8577 Japan
2 Satellite Venture Business Laboratory, Ehime University, 3 Bunkyo-cho, Matsuyama, Ehime, 790-8577 Japan
3 Center for Gene Research, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime, 790-8566 Japan
4 Graduate School of Science and Engineering, Ehime University, 2-5 Bunkyo-cho, Matsuyama, Ehime, 790-8577 Japan
5 National Institute of Advanced Industrial Science and Technology Kansai, 1-8-31 Midori-oka, Ikeda, Osaka, 563-8577 Japan
6 National Institute of Advanced Industrial Science and Technology Biological Information Research Center, AIST Waterfront 2-41-6 Aomi, Koto-ku, Tokyo, 135-0064 Japan

Abstract

SmtB is a trans-acting dimeric repressor of the metal-regulated smtA gene, and the release of SmtB from the smtA operator/promoter region is essential for the tolerance to Zn2+ stress by SmtA expression. Gel retardation assaying demonstrated that different sizes of SmtB–DNA complexes were formed depending on the DNA sequences, and the amounts of these complexes decreased in the presence of Zn2+. Here, we present the first direct evidence that Zn2+ (>4 µM) inhibits the SmtB–DNA complex formation in vitro, which ensures the physiological functions of SmtB as a Zn2+ sensor and a transcription factor.

Footnotes

7 Corresponding author: E-mail, ehmorita@dpc.ehime-u.ac.jp; Fax, +81-89-946-9968.


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