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Plant and Cell Physiology, 2002, Vol. 43, No. 10 1250-1253
© 2002 Oxford University Press

Short Communications

Cloning, Functional Expression, and Mutational Analysis of a cDNA for Lotus japonicus Mitochondrial Phosphate Transporter

Kazuki Nakamori1, Reona Takabatake2,4, Yosuke Umehara3, Hiroshi Kouchi3, Katsura Izui1,2 and Shingo Hata1,2,5

1 Laboratory of Plant Physiology, Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto, 606-8502 Japan
2 Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, 606-8502 Japan
3 National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, 305-8602 Japan

Abstract

A full-length cDNA encoding mitochondrial phosphate transporter was isolated from symbiotic nodules of Lotus japonicus, a model legume. The gene appeared to be single-copied and its transcript was abundant in roots, symbiotic root nodules and shoots, in that order. The L. japonicus transporter has a conserved N-ethylmaleimide-reactive Cys residue. Site-directed mutagenesis and phosphate transport assaying in vitro showed that this Cys residue is preferable for the transport activity, although it can be replaced to some extent by Ser, but not by Ala.

Footnotes

4 Present address: National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, 305-8602 Japan.

5 Corresponding author: E-mail, shing@kais.kyoto-u.ac.jp; Fax, +81-75-753-6470.


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