Skip Navigation

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (2)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Kwak, S.-H.
Right arrow Articles by Lee, S. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kwak, S.-H.
Right arrow Articles by Lee, S. H.
Agricola
Right arrow Articles by Kwak, S.-H.
Right arrow Articles by Lee, S. H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 2002, Vol. 43, No. 10 1165-1170
© 2002 Oxford University Press

The Transcript-Level-Independent Activation of Ornithine Decarboxylase in Suspension-Cultured BY2 Cells Entering the Cell Cycle

Su-Hwan Kwak and Sun Hi Lee1

Department of Biology, Yonsei University, Shinchon-dong 134, Seodaemun-gu, Seoul 120-749, Korea

The regulation of ornithine decarboxylase (ODC) expression was studied in suspension-cultured tobacco (Nicotiana tabacum L.) BY2 cells. ODC activity increased rapidly 3 h after cells re-entered the cell cycle from the stationary phase, corresponding to the G1 phase, and continued to increase in the subsequent S phase, while the ODC transcript level increased only transiently. ODC activity was suppressed by sucrose-deficiency, while the ODC transcript level was not affected. U0126, a specific inhibitor of mammalian MAPK kinases (MEKs), significantly reduced ODC enzyme activity, but not the ODC transcript level. These results suggest that ODC activity is regulated independently of its transcript level in BY2 cells, and that sucrose and a U0126-sensitive protein kinase are required for the transcript-level-independent activation of ODC.

1 Corresponding author: E-mail, shlee@biology.yonsei.ac.kr; Fax, +82-2-312-5657.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.