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Plant and Cell Physiology, 2002, Vol. 43, No. 10 1137-1145
© 2002 Oxford University Press

Salt-Tolerant ATPase Activity in the Plasma Membrane of the Marine Angiosperm Zostera marina L.

Yuki Muramatsu1, Akiko Harada2,5, Yoshinari Ohwaki3, Yoshiaki Kasahara4, Shingo Takagi2 and Toshiyuki Fukuhara1,6

1 Department of Applied Biological Sciences, Tokyo University of Agriculture and Technology, Saiwaicho 3-5-8, Fuchu, Tokyo, 183-8509 Japan
2 Department of Biology, Graduate School of Science, Osaka University, Machikaneyama 1-16, Toyonaka, Osaka, 560-0043 Japan
3 National Agricultural Research Center, Kannondai 3-1-1, Tsukuba, Ibaraki, 305-8666 Japan
4 Plant Nutrition Laboratory, National Agricultural Research Center for Hokkaido Region, 1 Hitsujigaoka, Toyohira-ku, Sapporo, 062-8555 Japan

Plasma membrane (PM) H+-ATPase and H+ transport activity were detected in PM fractions prepared from Zostera marina (a seagrass), Vallisneria gigantea (a freshwater grass) and Oryza sativa (rice, a terrestrial plant). The properties of Z. marina PM H+-ATPase, specifically, the optimal pH for ATPase activity and the result of trypsin treatment, were similar to those of authentic PM H+-ATPases in higher plants. In V. gigantea and O. sativa PM fractions, vanadate-sensitive (P-type) ATPase activities were inhibited by the addition of NaCl. In contrast, activity in the Z. marina PM fraction was not inhibited. The nitrate-sensitive (V-type) and azide-sensitive (F-type) ATPase activities in the Z. marina crude microsomal fraction and the cytoplasmic phosphoenolpyruvate carboxylase activity, however, were inhibited by NaCl, indicating that not all enzyme activities in Z. marina are insensitive to salt. Although the ratio of Na+ to K+ (Na+/K+) in seawater is about 30, Na+/K+ in the Z. marina cells was about 1.0. The salt-tolerant ATPase activity in the plasma membrane must play an important role in maintaining a low Na+ concentration in the seagrass cells.

5 Present address: Genomic Function Research Group, RIKEN Plant Science Center, Suehiro-cho 1-7-22, Tsurumi, Yokohama, Kanagawa, 230-0045 Japan.

6 Corresponding author: E-mail, fuku@cc.tuat.ac.jp; Fax, +81-42-360-8830.


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