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Plant and Cell Physiology, 2002, Vol. 43, No. 1 99-107
© 2002 Oxford University Press

Arabidopsis GARP Transcriptional Activators Interact with the Pro-Rich Activation Domain Shared by G-Box-Binding bZIP Factors

Hiroki Tamai, Masaki Iwabuchi1 and Tetsuo Meshi2

Department of Botany, Graduate School of Science, Kyoto University, Kyoto, 606-8502 Japan

The Pro-rich regions, found in a subset of plant bZIP transcription factors, including G-box-binding factors (GBFs) of Arabidopsis thaliana, are thought to be deeply involved in transcriptional regulation. However, the molecular mechanisms of the Pro-rich region-mediated transcriptional regulation are still largely unknown. Here we report evidence showing that two closely related Arabidopsis proteins, designated GPRI1 and GPRI2, containing a GARP DNA-binding domain, are likely partners of one or more GBFs. The results of yeast two-hybrid assays and in vitro binding assays indicated that GPRI1 can interact with the Pro-rich regions of GBF1 and GBF3. GPRI2 interacted with the Pro-rich region of GBF1. GPRI1 and GPRI2 transactivated transcription in yeast. In GPRI1 the region responsible for this activation was mapped in the N-terminal third of the protein. Transient assays showed that in Arabidopsis cells not only the N-terminal but also the C-terminal regions of GPRI1 can function as a separable activation domain. GPRI1 and GPRI2 may function in some promoters in concert with a GBF through interaction with its Pro-rich region to enhance the transcriptional level of the corresponding genes.

1 Present address: Research Institute for Biological Sciences (RIBS), 7459-1 Yoshikawa, Kayo-cho, Okayama, 716-1241 Japan.

2 Corresponding author: E-mail, tmeshi@gr.bot.kyoto-u.ac.jp; Fax, +81-75-753-4141.


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