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Plant and Cell Physiology, 2002, Vol. 43, No. 1 136-140
© 2002 Oxford University Press

Short Communications

Experimentally Determined Sequence Requirement of ACGT-Containing Abscisic Acid Response Element

Tsukaho Hattori1, Masako Totsuka, Tokunori Hobo, Yasuaki Kagaya and Akiko Yamamoto-Toyoda

Center for Molecular Biology and Genetics, Mie University, Tsu, 514-8507 Japan

Abstract

The sequence requirement of the ACGT-containing abscisic acid response element (ABRE) was analyzed by systematically substituting the bases surrounding the ACGT-core of motif A, the principal ABRE of the rice gene, Osem. This was done within the context of a 55-bp promoter fragment that minimally confers ABA-responsiveness to a heterologous promoter. Based on this analysis, the sequence requirement of the ACGT-containing ABRE was determined as ACGTG G/T C, which matched very well with the consensus derived from sequence comparison of ABA-responsive promoters.

Footnotes

1 Corresponding author: E-mail, hattori@gene.mie-u.ac.jp; Fax, +81-59-231-9048.


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