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Plant and Cell Physiology, 2001, Vol. 42, No. 9 952-958
© 2001 Oxford University Press

Distribution of Yieldin, a Regulatory Protein of the Cell Wall Yield Threshold, in Etiolated Cowpea Seedlings

Akane Okamoto-Nakazato1, Koji Takahashi3, Ritsuko Katoh-Semba2 and Kiyoshi Katou3,4

1 Biological Institute, Showa Pharmaceutical University, Higashitamagawa-gakuen 3-3165, Machida, Tokyo, 194-8543 Japan 2 Department of Perinatology, Institute for Developmental Research, Aichi Human Service Center, Kamiya-cho 713-8, Kasugai, Aichi, 480-0392 Japan 3 Unit of Biosystems, School of Informatics and Sciences and Graduate School of Human Informatics, Nagoya University, Nagoya, 464-8601 Japan

We examined the distribution and the immunohistochemical localization of yieldin in etiolated cowpea seedlings with an anti-yieldin antibody. An immunoblotting analysis revealed that the yieldin was located in the aerial organs (plumule, epicotyl and hypocotyl) but not in the roots. The intensity of the yieldin signal in the hypocotyls was highest in the apical pre-elongation region (the hook region) and decreased toward the elongated mature base indicating that the yieldin disappeared with the ceasing of cell elongation. Tissue-print immunoblotting analysis using hypocotyls in different germination stages supports this view because the apical yieldin-rich regions, just beneath the cotyledonary node (the hook and rapidly elongating regions), acropetally migrated together with hypocotyl elongation. Immunohistochemical microscopy demonstrated that yieldin was localized in the cell walls of the cortex and epidermis of the germ axes. The present results are consistent with the view that yieldin participates in the regulation of cell wall yielding during elongation growth.

4 Corresponding author: E-mail, j45810a@nucc.cc.nagoya-u.ac.jp; Fax, +81-52-789-4818.


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