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Plant and Cell Physiology, 2001, Vol. 42, No. 9 923-930
© 2001 Oxford University Press

Possible Involvement of Auxin-Induced Ethylene in an Apoptotic Cell Death during Temperature-Sensitive Lethality Expressed by Hybrid between Nicotiana glutinosa and N. repanda

Tetsuya Yamada1, Wataru Marubashi2,4, Teruko Nakamura3 and Masaru Niwa2

1 Plant Biotechnology Institute, Ibaraki Agricultural Center, Iwama, Ibaraki, 319-0292 Japan 2 School of Agriculture, Ibaraki University, Ami, Ibaraki, 300-0393 Japan 3 Faculty of Science, Japan Women’s University, Bunkyo, Tokyo, 112-8681 Japan

Interspecific hybrids of Nicotiana glutinosa L. x N. repanda Willd. express temperature-sensitive lethality induced by apoptotic cell death. Hybrid seedlings cultured at 28°C began to exhibit lethal symptoms during early growth stages, and then they showed a high level of endogenous auxin compared with those of parental seedlings. Meanwhile, the level of auxin in hybrid seedlings cultured at 32°C, which is a condition suppressing the lethality of this cross combination, was equal to or lower than those of parental seedlings. Administration of 2,3,5-triiodobenzoic acid (TIBA) as an auxin transport inhibitor into the hybrid seedlings suppressed lethal symptoms and had a life-extending effect. Additionally, TIBA has an effect to suppress DNA fragmentation, which is one of characteristics of apoptosis and has been detected in the hybrid seedlings expressing the lethality. Administration of aminooxyacetic acid (AOA) as an ethylene synthesis inhibitor, which could inhibit ethylene production, also showed the same effects as TIBA for the lethality. From these results, we suggested that auxin and ethylene were involved in an apoptotic cell death during the lethality, and the abnormal increase of endogenous auxin may lead to the ethylene production in hybrid seedlings during early growth stages.

4 Corresponding author: E-mail, marubasi@ipc.ibaraki.ac.jp; Fax, +81-298-88-8644.


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