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Plant and Cell Physiology, 2001, Vol. 42, No. 8 879-883
© 2001 Oxford University Press

Redox Changes Accompanying the Degradation of Seed Storage Proteins in Germinating Rice

Hiroyuki Yano1, Joshua H. Wong, Myeong-Je Cho and Bob B. Buchanan2

Department of Plant and Microbial Biology, University of California, 111 Koshland Hall, Berkeley, CA 94720, U.S.A.

The mobilization of storage proteins (glutelins) in germinating rice seeds was accompanied by an ordered sequential combination of proteolysis and reduction of disulfide groups. Mobilization was followed by application of non-reducing/reducing two dimensional-PAGE after monobromobimane labeling of the sulfhydryl groups of the proteins in intact seeds.

1 Present address: Department of Rice Research, National Agricultural Research Organization, Inada 1-2-1, Joetsu, Niigata, 943-0193 Japan.

2 Corresponding author: E-mail, view@nature.berkeley.edu; Fax: +1-510-642-7356; Phone, +1-510-642-3590.


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