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Plant and Cell Physiology, 2001, Vol. 42, No. 8 873-878
© 2001 Oxford University Press

Localization of CYP86B1 in the Outer Envelope of Chloroplasts

Christy J. W. Watson1, John E. Froehlich2, Caroline A. Josefsson1, Clint Chapple3, Francis Durst4, Irene Benveniste4 and Ronald C. Coolbaugh ,1,5

1 Department of Botany and Plant Pathology, Purdue University, W. Lafayette, IN 47907, U.S.A. 2 MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, MI, U.S.A. 3 Department of Biochemistry, Purdue University, W. Lafayette, IN 47907, U.S.A. 4 Department d’Enzymologie Cellulaire et Moleculaire, Institut de Biologie Moleculaire des Plantes, CNRS, 28 rue Goethe, F-67000 Strasbourg, France

CYP86B1 was cloned from a cDNA library and the protein expressed in E. coli. The protein gave the expected carbon monoxide difference spectrum. Using in vitro import assays with isolated pea chloroplasts, CYP86B1 was shown to be associated with the outer chloroplastic envelope membrane. This study provides the first direct evidence for a chloroplast-localized cytochrome P450-dependent monooxygenase.

5 Corresponding author: Email, coolbaugh@purdue.edu; Fax, +1-765-494-5896.


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