Skip Navigation

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (13)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Fukao, Y.
Right arrow Articles by Nishimura, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fukao, Y.
Right arrow Articles by Nishimura, M.
Agricola
Right arrow Articles by Fukao, Y.
Right arrow Articles by Nishimura, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 2001, Vol. 42, No. 8 835-841
© 2001 Oxford University Press

Developmental Analysis of a Putative ATP/ADP Carrier Protein Localized on Glyoxysomal Membranes During the Peroxisome Transition in Pumpkin Cotyledons

Youichiro Fukao1,2, Yasuko Hayashi3, Shoji Mano1, Makoto Hayashi1 and Mikio Nishimura ,1,2,4

1 Departmnt of Cell Biology, National Institute for Basic Biology, Okazaki, 444-8585 Japan 2 Department of Molecular Biomechanics, School of Life Science, Graduate University for Advanced Studies, Okazaki, 444-8585 Japan 3 Departmnt of Environmental Science, Faculty of Science, Niigata University, Ikarashi, Niigata, 950-2181 Japan

In order to clarify the peroxisomal membrane proteins (PMPs), we characterized one of the major PMPs, PMP38. The deduced amino acid sequence for its cDNA in Arabidopsis thaliana contained polypeptides with 331 amino acids and had high similarity with those of Homo sapiens PMP34 and Candida boidinii PMP47 known as homologues of mitochondrial ATP/ADP carrier protein. We expected PMP38 to be localized on peroxisomal membranes, because it had the membrane peroxisomal targeting signal. Cell fractionation and immunocytochemical analysis using pumpkin cotyledons revealed that PMP38 is localized on peroxisomal membranes as an integral membrane protein. The amount of PMP38 in pumpkin cotyledons increased and reached the maximum protein level after 6 d in the dark but decreased thereafter. Illumination of the seedlings caused a significant decrease in the amount of the protein. These results clearly showed that the membrane protein PMP38 in glyoxysomes changes dramatically during the transformation of glyoxysomes to leaf peroxisomes, as do the other glyoxysomal enzymes, especially enzymes of the fatty acid ß-oxidation cycle, that are localized in the matrix of glyoxysomes.

4 Corresponding author: E-mail, mikosome@nibb.ac.jp; Fax, +81-564-55-7505.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant CellHome page
N. Linka, F. L. Theodoulou, R. P. Haslam, M. Linka, J. A. Napier, H. E. Neuhaus, and A. P.M. Weber
Peroxisomal ATP Import Is Essential for Seedling Development in Arabidopsis thaliana
PLANT CELL, December 1, 2008; 20(12): 3241 - 3257.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
Y. Arai, M. Hayashi, and M. Nishimura
Proteomic Identification and Characterization of a Novel Peroxisomal Adenine Nucleotide Transporter Supplying ATP for Fatty Acid {beta}-Oxidation in Soybean and Arabidopsis
PLANT CELL, December 1, 2008; 20(12): 3227 - 3240.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
H. Eubel, E. H. Meyer, N. L. Taylor, J. D. Bussell, N. O'Toole, J. L. Heazlewood, I. Castleden, I. D. Small, S. M. Smith, and A. H. Millar
Novel Proteins, Putative Membrane Transporters, and an Integrated Metabolic Network Are Revealed by Quantitative Proteomic Analysis of Arabidopsis Cell Culture Peroxisomes
Plant Physiology, December 1, 2008; 148(4): 1809 - 1829.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
T. Kamada, K. Nito, H. Hayashi, S. Mano, M. Hayashi, and M. Nishimura
Functional Differentiation of Peroxisomes Revealed by Expression Profiles of Peroxisomal Genes in Arabidopsis thaliana
Plant Cell Physiol., December 15, 2003; 44(12): 1275 - 1289.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
Y. Fukao, M. Hayashi, I. Hara-Nishimura, and M. Nishimura
Novel Glyoxysomal Protein Kinase, GPK1, Identified by Proteomic Analysis of Glyoxysomes in Etiolated Cotyledons of Arabidopsis thaliana
Plant Cell Physiol., October 15, 2003; 44(10): 1002 - 1012.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
H. Hayashi, L. De Bellis, Y. Hayashi, K. Nito, A. Kato, M. Hayashi, I. Hara-Nishimura, and M. Nishimura
Molecular Characterization of an Arabidopsis Acyl-Coenzyme A Synthetase Localized on Glyoxysomal Membranes
Plant Physiology, December 1, 2002; 130(4): 2019 - 2026.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
Y. Fukao, M. Hayashi, and M. Nishimura
Proteomic Analysis of Leaf Peroxisomal Proteins in Greening Cotyledons of Arabidopsis thaliana
Plant Cell Physiol., July 15, 2002; 43(7): 689 - 696.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
S. Mano, C. Nakamori, M. Hayashi, A. Kato, M. Kondo, and M. Nishimura
Distribution and Characterization of Peroxisomes in Arabidopsis by Visualization with GFP: Dynamic Morphology and Actin-Dependent Movement
Plant Cell Physiol., March 1, 2002; 43(3): 331 - 341.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.