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Plant and Cell Physiology, 2001, Vol. 42, No. 6 620-626
© 2001 Oxford University Press

Different Cytoplasmic Calcium Contents Among Three Species of Characeae

Masashi Tazawa ,1,4, Munehiro Kikuyama2 and Yoshiji Okazaki3 1 Department of Applied Physics and Chemistry, Fukui University of Technology, Fukui, 910-8505 Japan 2 Laboratory of Biology, University of the Air, Chiba, 261-0014 Japan 3 Department of Biology, Osaka Medical College, Osaka, 569-0084 Japan

Internodal cells of three species of Characeae, Nitella flexilis, Nitella axilliformis and Chara corallina, were analyzed for the contents of Ca2+ and Mg2+ in the cytoplasm. To avoid contamination of Ca2+ from the cell wall and vacuole, the vacuolar sap was replaced with a sorbitol solution containing Sr2+ by the vacuolar perfusion method after the cell had been treated with Sr2+. No significant difference in the cytoplasmic content of Mg2+ was found among three species of Characeae, but significant differences in the cytoplasmic content of Ca2+ were observed among them. The cytoplasmic Ca2+ content of N. flexilis was 2.0 times that of N. axilliformis and 3.3 times that of C. corallina. The cytoplasmic drop was furthermore separated into two fractions: a chloroplast-free fraction and a chloroplast fraction. In the chloroplast-free fraction the Ca2+ content of N. flexilis was 2.3 times that of C. corallina and 2.0 times that of N. axilliformis, while the Mg2+ content was the same among the three species. In the chloroplast fraction N. flexilis contained about seven times more Ca2+ and about two times more Mg2+ than C. corallina. The difference in the cytoplasmic Ca2+ content was discussed in relation to the difference in the capacity for the hydration-induced Ca2+ release existing among the three species.

4 Corresponding author: E-mail, Masashi.Tazawa@mb5.seikyou.ne.jp; Fax, +81-77-524-9221.


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