Plant and Cell Physiology, 2001, Vol. 42, No. 5 524-530
© 2001 Oxford University Press
Association of Glutathione with Flowering in Arabidopsis thaliana
1 Research Institute for Biological Sciences, Okayama (RIBS), 7549-1 Yoshikawa, Kayou-cho, Okayama, 716-1241 Japan
In order to study the relationship between GSH and flowering, wild-type and late-flowering mutant, fca-1, of Arabidopsis thaliana were treated with L-buthionine sulfoximine (BSO), a specific inhibitor of GSH biosynthesis, under long-day conditions. BSO treatment of the fca-1 mutant starting at 17 d after imbibition promoted flowering. However, when the treatment was started at 12 d after imbibition, BSO treatment at 10–4 M resulted in an inhibition of flowering. This inhibitory effect of BSO on flowering was abolished by GSH treatment at 10–4 M, although GSH treatment at an increased concentration of 10–3 M clearly delayed flowering. In contrast, BSO treatment of wild-type plants starting at 12 d after imbibition promoted flowering, whose effect was abolished by GSH application. In the fca-1 mutant, whose endogenous GSH levels were high, chilling treatment lowered the GSH levels and promoted flowering, as was the case in the BSO treatment. An A. thaliana mutant, cad2-1, which has a defect in GSH biosynthesis also exhibited late flowering. The late-flowering phenotype of this mutant tended to be strengthened by BSO and abolished by GSH treatment. These results suggest that flowering is associated with the rate of GSH biosynthesis and/or the levels of GSH in A. thaliana.
2 Corresponding author: E-mail, ogawa_k@bc4.so-net.ne.jp; Fax, +81-866-56-9454.
3 The first two authors equally contributed to the present work.
4 Current address: Institute of Biological Resources, National Institute of Advanced Industrial Science and Technology (AIST), 17-2-1 Tsukisamu-Higashi-Nijo, Sapporo, Hokkaido, 062-8517 Japan.
5 Current address: Department of Agricultural Science, Faculty of Agriculture, Kyoto Prefectural University, Kyoto, 606-8522 Japan.
6 Current address: Department of Molecular Genetics, National Institute of Agrobiological Resources, 2-1-2 Kan-nondai, Tsukuba, Ibaraki, 305-8602 Japan.
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