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Plant and Cell Physiology, 2001, Vol. 42, No. 3 264-273
© 2001 Oxford University Press

The Chloroplast clpP Gene, Encoding a Proteolytic Subunit of ATP-Dependent Protease, is Indispensable for Chloroplast Development in Tobacco

Toshiharu Shikanai ,1,3, Katsumi Shimizu1, Katsumi Ueda1, Yoshiki Nishimura2, Tsuneyoshi Kuroiwa2 and Takashi Hashimoto1

1 Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, 630-0101 Japan 2 Department of Biological Sciences, Graduate School of Sciences, University of Tokyo, Hongo, Bunkyo-ku, Tokyo, 113-0033 Japan

ClpP is a proteolytic subunit of the ATP-dependent Clp protease, which is found in chloroplasts in higher plants. Proteolytic subunits are encoded both by the chloroplast gene, clpP, and a nuclear multi gene family. We insertionally disrupted clpP by chloroplast transformation in tobacco. However, complete segregation was impossible, indicating that the chloroplast-encoded clpP gene has an indispensable function for cell survival. In the heteroplasmic clpP disruptant, the leaf surface was rough by clumping, and the lateral leaf expansion was irregularly arrested, which led to an asymmetric, slender leaf shape. Chloroplasts consisted of two populations: chloroplasts that were similar to the wild type, and small chloroplasts that emitted high chl fluorescence. Ultrastructural analysis of chloroplast development suggested that clpP disruption also induced swelling of the thylakoid lumen in the meristem plastids and inhibition of etioplast development in the dark. In mature leaves, thylakoid membranes of the smaller chloroplast population consisted exclusively of large stacks of tightly appressed membranes. These results indicate that chloroplast-encoded ClpP is involved in multiple processes of chloroplast development, including a housekeeping function that is indispensable for cell survival.

3 Corresponding author: E-mail, shikanai@bs.aist-nara.ac.jp; Fax, +81-0743-72-5489.


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