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Plant and Cell Physiology, 2001, Vol. 42, No. 2 189-196
© 2001 Oxford University Press

Physical Arrangement of Retrotransposon-Related Repeats in Centromeric Regions of Wheat

Kazu-Nori Fukui1, Go Suzuki1, Evans S. Lagudah2, Sadequr Rahman2, Rudi Appels2, Maki Yamamoto3 and Yasuhiko Mukai ,1,4

1 Division of Natural Science, Osaka Kyoiku University, Kashiwara, 582-8582 Japan 2 Commonwealth Scientific and Industrial Research Organization (CSIRO), Plant Industry, Canberra ACT 2601, Australia 3 Kansai Women’s College, Kashiwara, 582-0026 Japan

Cereal centromeres commonly contain many repetitive sequences that are derived from Ty3/gypsy retrotransposon. FISH analysis using a large DNA insert library of wheat identified a 67-kb clone (R11H) that showed strong hybridization signals on the centromeres. The R11H clone contains Ty3/gypsy retrotransposon-related sequences; both integrase and CCS1 family sequences were identified. Subsequently, we isolated additional 23 large-insert clones which also contained the integrase and CCS1 sequences. Based on the number of the integrase repeats in the clones determined by DNA gel blot analysis, we concluded that the retrotransposon-like sequences are tandemly repeated in wheat centromeres in ca. 55-kb interval on average. This conclusion is consistent with the results of FISH analysis on the extended DNA fibers.

4 Corresponding author: E-mail, ymukai@cc.osaka-kyoiku.ac.jp; Fax, +81-729-78-3660.


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