Skip Navigation

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (18)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Shen, Y.
Right arrow Articles by Christopher, D. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shen, Y.
Right arrow Articles by Christopher, D. A.
Agricola
Right arrow Articles by Shen, Y.
Right arrow Articles by Christopher, D. A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 2001, Vol. 42, No. 10 1071-1078
© 2001 Oxford University Press

RNA Binding-Proteins Interact Specifically with the Arabidopsis Chloroplast psbA mRNA 5' Untranslated Region in a Redox-Dependent Manner

Yanxin Shen1, Avihai Danon2 and David A. Christopher1

1 Department of Molecular Biosciences and Biosystems Engineering, University of Hawaii, 1955 East-West Road, Honolulu, HI 96822 U.S.A. 2 Department of Plant Sciences, Weizmann Institute of Science, P.O.B. 26, Rehovot, Israel

The 5' untranslated region (5'UTR) of the psbA mRNA (psbA encodes the PSII reaction center protein, D1) is a key site for RNA–protein interactions in the post-transcriptional regulation of gene expression. In this study, we mapped the major psbA mRNA 5'-terminus at –77 nt, and two minor termini clusters centered at –48 and –64 nt, upstream from the psbA translational start codon of Arabidopsis thaliana. RNA mobility shift, RNase protection and UV-crosslinking assays were used to characterize the interaction of chloroplast proteins with the RNA 5'UTR. RNA–protein interactions depended upon a thermolabile secondary structure and specific sequences in a 35 nt region of the 5'UTR, which were 80% conserved with the psbA 5'UTRs from five other plants. Major and minor proteins of 43- and 30-kDa, respectively, were detected by UV-crosslinking to RNA. Oxidizing conditions abolished the association of the proteins with the 5'UTR, while RNA-binding activity was recovered upon incubation with a reductant. Based on these findings, we hypothesize that post-transcriptional regulation of psbA gene expression in chloroplasts of vascular plants involves redox-dependent interactions between specific sequences in the 5'UTR and 43- and 30-kDa RNA-binding proteins.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 ANN BOT (LOND)Home page
T. Pfannschmidt, K. Brautigam, R. Wagner, L. Dietzel, Y. Schroter, S. Steiner, and A. Nykytenko
Potential regulation of gene expression in photosynthetic cells by redox and energy state: approaches towards better understanding
Ann. Bot., February 1, 2009; 103(4): 599 - 607.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
K. Schult, K. Meierhoff, S. Paradies, T. Toller, P. Wolff, and P. Westhoff
The Nuclear-Encoded Factor HCF173 Is Involved in the Initiation of Translation of the psbA mRNA in Arabidopsis thaliana
PLANT CELL, April 1, 2007; 19(4): 1329 - 1346.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. Fey, R. Wagner, K. Brautigam, M. Wirtz, R. Hell, A. Dietzmann, D. Leister, R. Oelmuller, and T. Pfannschmidt
Retrograde Plastid Redox Signals in the Expression of Nuclear Genes for Chloroplast Proteins of Arabidopsis thaliana
J. Biol. Chem., February 18, 2005; 280(7): 5318 - 5328.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
R. G. Walters
Towards an understanding of photosynthetic acclimation
J. Exp. Bot., January 1, 2005; 56(411): 435 - 447.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
L. Tang, S. Bhat, and M. E. Petracek
Light Control of Nuclear Gene mRNA Abundance and Translation in Tobacco
Plant Physiology, December 1, 2003; 133(4): 1979 - 1990.
[Abstract] [Full Text]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.