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Plant and Cell Physiology, 2001, Vol. 42, No. 10 1049-1055
© 2001 Oxford University Press

Extracellular Calmodulin Stimulates Light-Independent RbcS-GUS Expression in Suspension-Cultured Cells of Transgenic Tobacco

Junli Zhou1, Ligeng Ma1, Suqiao Zhang1, Yuxian Zhu2 and Daye Sun1,3

1 Institute of Molecular Cell Biology, Hebei Normal University, Shijiazhuang, Hebei 050016, P. R. of China 2 College of Life Science, Peking University, Beijing 100087, P. R. of China

The RbcS genes encode the small subunits of rubisco; the expression of these genes is controlled in a light-dependent and independent manner. It has been reported that intracellular calmodulin (CaM) is involved in light-dependent RbcS expression. In this report, the role of extracellular CaM in regulating expression of RbcS in darkness was examined. The time course of expression of RbcS-GUS and that of the secretion of CaM in the suspended transgenic tobacco cells in darkness were very similar. Both showed initial increase followed by decline with maximum CaM secretion preceding maximum GUS expression by 24 h. The concentration of CaM in the culture medium is regulated light independently. Purified CaM alone added to the media enhanced RbcS-GUS expression in darkness. The addition of membrane-impermeable CaM inhibitors, such as anti-CaM antiserum or W7-agarose, repressed the expression of RbcS-GUS in darkness, but this inhibitory effect was completely reversed by adding exogenous purified CaM. These results provide the first evidence that extracellular CaM is involved in the regulation of light-independent RbcS gene expression.

3 Corresponding author: E-mail, sundaye@public.sj.he.cn; Fax, +86-311-5820649; Phone, +86-311-5820649.


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