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Plant and Cell Physiology, 2001, Vol. 42, No. 1 54-62
© 2001 Oxford University Press

Biochemical Characterization of Profilin from Seeds of Phaseolus vulgaris L.

Gabriel Guillén, Lorena M.L. López-Sánchez, Carolina San Román-Roque, Federico Sánchez and Marco A. Villanueva1

Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, U.N.A.M. Apartado Postal 510-3, Cuernavaca, Morelos 62250, México

The isoform composition of the 14.4 kDa profilin polypeptide was analyzed in seeds, leaves, flowers, roots and root-nodules from Phaseolus vulgaris L. Isoforms of pIs ~4.4–5 were present in all the tissues analyzed. The biochemical features of the protein present in seed tissue were determined. Seed profilin bound to Phenyl-Sepharose under low salt conditions which suggested a hydrophobic interaction; however, it was not associated with microsomal membranes nor it partitioned as a hydrophobic protein in Triton X-114. Fractions eluting from poly-L-proline or Phenyl-Sepharose columns contained well detectable amounts of profilin but no actin, suggesting that most of the protein was not present as profilactin in the seed. However, seed profilin appeared to be in some kind of complex since several molecular weight species were observed on native gels. In addition, profilin was found preferentially in the embryo axis and light microscopic immunolocalization showed a cytoplasmic distribution in this tissue.

1 Corresponding author: E-mail, marco@ibt.unam.mx; Fax, +52-73-13-99-88.


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