Plant and Cell Physiology, 2000, Vol. 41, No. 8 948-959
© 2000 Oxford University Press
Highly Substituted Glucuronoarabinoxylans (hsGAXs) and Low-Branched Xylans Show a Distinct Localization Pattern in the Tissues of Zea mays L.
1 Wood Research Institute, Kyoto University, Uji, Kyoto, 611-0011 Japan 2 Department of Biological Resource Chemistry, Kyoto Prefectural University, Shimogamo, Kyoto, 606-8522 Japan 3 Faculty of Education, Hirosaki University, Bunkyo cho-1, Hirosaki, 036-8560 Japan
Polyclonal antibodies which recognized highly substituted glucuronoarabinoxylans (hsGAXs) and low-branched xylans and did not cross-react with each other, were raised in order to examine localization of these epitopes in internodes of maize. Immunofluorescent labeling revealed different pattern between two succeeding developmental stages. The hsGAX epitope was localized evenly in primary walls in all tissue types, and strongly in unlignified secondary walls in phloem. However, lignified secondary walls in protoxylem, parenchyma and a part of fibers were faintly labeled with this epitope. Moreover, the epitope showed limited binding in lignified parenchyma and fiber walls at ultrastructural level. Low-branched xylan epitope was localized evenly throughout lignified walls in all tissue types. This epitope was also localized only in lignified walls of other organs such as leaf, root apex and dark-grown mesocotyl. Low-branched xylans are significantly related to lignification. Localization of hsGAX epitope in their organs was similar to that in internodes. The hsGAX epitope was distributed both in unlignified walls of all tissues and in lignified walls of parenchyma and annular thickening of protoxylem. We propose that hsGAX has separate functions in lignified and unlignified tissues. In conclusion, at tissue level, hsGAX is localized mainly in unlignified walls, and low-branched xylans in lignified walls.
4 Corresponding author: E-mail, titoh@kuwri.kyoto-u.ac.jp; Fax, +81-774-38-3635.
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