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Plant and Cell Physiology, 2000, Vol. 41, No. 8 920-931
© 2000 Oxford University Press

Isolation of a Novel 190 kDa Protein from Tobacco BY-2 Cells: Possible Involvement in the Interaction between Actin Filaments and Microtubules

Hisako Igarashi1, Hidefumi Orii1, Hitoshi Mori2, Teruo Shimmen1 and Seiji Sonobe1,3

1 Department of Life Science, Faculty of Science, Himeji Institute of Technology, Harima Science Park City, Hyogo, 678-1297 Japan 2 School of Agriculture, Nagoya University, Chikusa, Nagoya, 464 Japan

Interaction between actin filaments (AFs) and microtubules (MTs) has been reported in various plant cells, and the presence of a factor(s) connecting these two cytoskeletal networks has been suggested, but its molecular entity has not been elucidated yet. We obtained a fraction containing MT-binding polypeptides, which induced bundling of AFs and of MTs. A 190 kDa polypeptide which associated with AFs was selectively isolated from the fraction. This polypeptide was thought to have an ability to bind to both AFs and MTs. We raised a monoclonal antibody against the 190 kDa polypeptide. Immunostaining demonstrated the association of the 190 kDa polypeptide with AF bundles and with MT bundles formed in vitro. Immunocytochemical studies throughout the cell cycle revealed that the 190 kDa polypeptide was localized in the nucleus before nuclear envelope breakdown, and in the spindle and the phragmoplast during cell division. After the re-formation of the nuclear envelope, the 190 kDa polypeptide was sequestered to the daughter nuclei. Using the antibody, we succeeded in cloning a cDNA encoding the 190 kDa polypeptide.

3 Corresponding author: E-mail, sonobe@sci.himeji-tech.ac.jp; Fax, +81-791-58-0175.


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