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Plant and Cell Physiology, 2000, Vol. 41, No. 12 1334-1346
© 2000 Oxford University Press

The YELLOW VARIEGATED (VAR2) Locus Encodes a Homologue of FtsH, an ATP-Dependent Protease in Arabidopsis

Katsuaki Takechi, Sodmergen1, Minoru Murata, Fusao Motoyoshi and Wataru Sakamoto2

Research Institute for Bioresources, Okayama University, 2-20-1 Chuo, Kurashiki, Okayama, 710-0046 Japan

Variegated leaves are often caused by a nuclear recessive mutation in higher plants. Characterization of the gene responsible for variegation has shown to provide several pathways involved in plastid differentiation. Here we describe an Arabidopsis variegated mutant isolated by T-DNA tagging. The mutant displayed green and yellow sectors in all green tissues except for cotyledons. Cells in the yellow sector of the mutant contained both normal-appearing and mutant chloroplasts. The isolated mutant was shown to be an allele of the previously reported mutant, yellow variegated (var2). Cloning and molecular characterization of the VAR2 locus revealed that it potentially encodes a chloroplastic homologue of FtsH, an ATP-dependent metalloprotease that belongs to a large protein family involved in various cellular functions. ftsH-like genes appear to comprise a small gene family in Arabidopsis genome, since at least six homologues were found in addition to VAR2. Dispensability of VAR2 was therefore explained by the redundancy of genes coding for FstHs. In the yellow regions of the mutant leaves, accumulation of photosynthetic protein components in the thylakoid membrane appeared to be impaired. Based on the role of FtsH in a protein degradation pathway in plastids, we propose a possibility that VAR2 is required for plastid differentiation by avoiding partial photooxidation of developing chloroplasts.

1 Permanent address: College of Life Sciences, Peking University, Beijing 100871, China.

2 Correspoding author: E-mail, saka@rib.okayama-u.ac.jp; Fax, +81-86-434-1208; Phone, +81-86-434-1206.


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