Plant and Cell Physiology, 2000, Vol. 41, No. 12 1299-1304
© 2000 Oxford University Press
Paper |
Novel Putative Photoreceptor and Regulatory Genes Required for the Positive Phototactic Movement of the Unicellular Motile Cyanobacterium Synechocystis sp. PCC 6803
1 Department of Life Sciences (Biology), University of Tokyo, Komaba 3-8-1, Meguro, Tokyo, 153-8902 Japan 2 Department of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa, Nagoya, 464-8602 Japan
Abstract
Synechocystis sp. PCC 6803 is a unicellular motile cyanobacterium, which shows positive or negative phototaxis on agar plates under lateral illumination. By gene disruption in a substrain showing of positive phototaxis, it was demonstrated that mutants defective in sll0038, sll0039, sll0041, sll0042 or sll0043 lost positive phototaxis but showed negative phototaxis away from the light source. Mutants of sll0040, which is located within the cluster of these genes, retained the capacity of positive phototaxis but to a lesser extent than the parent cells. These genes are homologous to che genes, which are involved in flagellar switching for bacterial chemotaxis. Interestingly, sll0041 (designated pisJ1) is predicted to have a chromophore-binding motif of phytochrome-like proteins and a signaling motif of chemoreceptors for bacterial chemotaxis. It is strongly suggested that the positive phototactic response was mediated by a phytochrome-like photoreceptor and CheA/CheY-type signal transduction system.
Footnotes
3 Corresponding author: E-mail, mikeuchi@ims.u-tokyo.ac.jp; Fax, +81-3-5454-4337.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  A. T. Ulijasz, G. Cornilescu, D. von Stetten, C. Cornilescu, F. Velazquez Escobar, J. Zhang, R. J. Stankey, M. Rivera, P. Hildebrandt, and R. D. Vierstra Cyanochromes Are Blue/Green Light Photoreversible Photoreceptors Defined by a Stable Double Cysteine Linkage to a Phycoviolobilin-type Chromophore J. Biol. Chem., October 23, 2009; 284(43): 29757 - 29772. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Jekely Evolution of phototaxis Phil Trans R Soc B, October 12, 2009; 364(1531): 2795 - 2808. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Asayama and S. Imamura Stringent promoter recognition and autoregulation by the group 3 {sigma}-factor SigF in the cyanobacterium Synechocystis sp. strain PCC 6803 Nucleic Acids Res., September 1, 2008; 36(16): 5297 - 5305. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Hirose, T. Shimada, R. Narikawa, M. Katayama, and M. Ikeuchi Cyanobacteriochrome CcaS is the green light receptor that induces the expression of phycobilisome linker protein PNAS, July 15, 2008; 105(28): 9528 - 9533. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Ishizuka, R. Narikawa, T. Kohchi, M. Katayama, and M. Ikeuchi Cyanobacteriochrome TePixJ of Thermosynechococcus elongatus Harbors Phycoviolobilin as a Chromophore Plant Cell Physiol., September 1, 2007; 48(9): 1385 - 1390. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Nakasugi, C. J. Svenson, and B. A. Neilan The competence gene, comF, from Synechocystis sp. strain PCC 6803 is involved in natural transformation, phototactic motility and piliation Microbiology, December 1, 2006; 152(12): 3623 - 3631. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Ishizuka, T. Shimada, K. Okajima, S. Yoshihara, Y. Ochiai, M. Katayama, and M. Ikeuchi Characterization of Cyanobacteriochrome TePixJ from a Thermophilic Cyanobacterium Thermosynechococcus elongatus Strain BP-1 Plant Cell Physiol., September 1, 2006; 47(9): 1251 - 1261. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. K. Ashby and J. Houmard Cyanobacterial Two-Component Proteins: Structure, Diversity, Distribution, and Evolution Microbiol. Mol. Biol. Rev., June 1, 2006; 70(2): 472 - 509. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. S. Makarova, E. V. Koonin, R. Haselkorn, and M. Y. Galperin Cyanobacterial response regulator PatA contains a conserved N-terminal domain (PATAN) with an alpha-helical insertion Bioinformatics, June 1, 2006; 22(11): 1297 - 1301. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-i. Maeda, C. Sugita, M. Sugita, and T. Omata Latent Nitrate Transport Activity of a Novel Sulfate Permease-like Protein of the Cyanobacterium Synechococcus elongatus J. Biol. Chem., March 3, 2006; 281(9): 5869 - 5876. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Giraud, S. Zappa, L. Vuillet, J.-M. Adriano, L. Hannibal, J. Fardoux, C. Berthomieu, P. Bouyer, D. Pignol, and A. Vermeglio A New Type of Bacteriophytochrome Acts in Tandem with a Classical Bacteriophytochrome to Control the Antennae Synthesis in Rhodopseudomonas palustris J. Biol. Chem., September 16, 2005; 280(37): 32389 - 32397. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Okajima, S. Yoshihara, Y. Fukushima, X. Geng, M. Katayama, S. Higashi, M. Watanabe, S. Sato, S. Tabata, Y. Shibata, et al. Biochemical and Functional Characterization of BLUF-Type Flavin-Binding Proteins of Two Species of Cyanobacteria J. Biochem., June 1, 2005; 137(6): 741 - 750. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Yoshihara, M. Katayama, X. Geng, and M. Ikeuchi Cyanobacterial Phytochrome-like PixJ1 Holoprotein Shows Novel Reversible Photoconversion Between Blue- and Green-absorbing Forms Plant Cell Physiol., December 15, 2004; 45(12): 1729 - 1737. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kobayashi, T. Ishizuka, M. Katayama, M. Kanehisa, M. Bhattacharyya-Pakrasi, H. B. Pakrasi, and M. Ikeuchi Response to Oxidative Stress Involves a Novel Peroxiredoxin Gene in the Unicellular Cyanobacterium Synechocystis sp. PCC 6803 Plant Cell Physiol., March 15, 2004; 45(3): 290 - 299. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W.-O. Ng, A. R. Grossman, and D. Bhaya Multiple Light Inputs Control Phototaxis in Synechocystis sp. Strain PCC6803 J. Bacteriol., March 1, 2003; 185(5): 1599 - 1607. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Kondou, N. Mogami, F. Hoshi, S. Kutsuna, M. Nakazawa, T. Sakurai, M. Matsui, T. Kaneko, S. Tabata, I. Tanaka, et al. Bipolar Localization of Putative Photoreceptor Protein for Phototaxis in Thermophilic Cyanobacterium Synechococcus elongatus Plant Cell Physiol., December 15, 2002; 43(12): 1585 - 1588. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Yoshihara, X. Geng, and M. Ikeuchi pilG Gene Cluster and Split pilL Genes Involved in Pilus Biogenesis, Motility and Genetic Transformation in the Cyanobacterium Synechocystis sp. PCC 6803 Plant Cell Physiol., May 15, 2002; 43(5): 513 - 521. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Alexandre and I. B. Zhulin More Than One Way To Sense Chemicals J. Bacteriol., August 15, 2001; 183(16): 4681 - 4686. [Full Text] [PDF]
|
|
|
|
|
|
D. Bhaya, A. Takahashi, and A. R. Grossman Light regulation of type IV pilus-dependent motility by chemosensor-like elements in Synechocystis PCC6803 PNAS, June 7, 2001; (2001) 131201098. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kamei, T. Yuasa, K. Orikawa, X. X. Geng, and M. Ikeuchi A Eukaryotic-Type Protein Kinase, SpkA, Is Required for Normal Motility of the Unicellular Cyanobacterium Synechocystis sp. Strain PCC 6803 J. Bacteriol., March 1, 2001; 183(5): 1505 - 1510. [Abstract] [Full Text]
|
|
|
|
|
|
S. Yoshihara, X. X. Geng, S. Okamoto, K. Yura, T. Murata, M. Go, M. Ohmori, and M. Ikeuchi Mutational Analysis of Genes Involved in Pilus Structure, Motility and Transformation Competency in the Unicellular Motile CyanobacteriumSynechocystis sp. PCC 6803 Plant Cell Physiol., January 1, 2001; 42(1): 63 - 73. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Bhaya, A. Takahashi, and A. R. Grossman Light regulation of type IV pilus-dependent motility by chemosensor-like elements in Synechocystis PCC6803 PNAS, June 19, 2001; 98(13): 7540 - 7545. [Abstract] [Full Text] [PDF]
|
|