Skip Navigation

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (19)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Kotake, T.
Right arrow Articles by Sakurai, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kotake, T.
Right arrow Articles by Sakurai, N.
Agricola
Right arrow Articles by Kotake, T.
Right arrow Articles by Sakurai, N.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 2000, Vol. 41, No. 11 1272-1278
© 2000 Oxford University Press

Auxin-Induced Elongation Growth and Expressions of Cell Wall-Bound Exo- and Endo-ß-Glucanases in Barley Coleoptiles

Toshihisa Kotake1, Naoki Nakagawa1, Kazuyoshi Takeda2 and Naoki Sakurai1,3

1 Department of Environmental Studies, Faculty of Integrated Arts & Sciences, Hiroshima University, Higashi-Hiroshima, 739-8521 Japan 2 Research Institute for Bioresources, Okayama University, Kurashiki, Okayama, 710-0046 Japan

When auxin stimulates rapid cell elongation growth of cereal coleoptiles, it causes a degradation of 1,3:1,4-ß-glucan in hemicellulosic polysaccharides. We examined gene expressions of endo-1,3:1,4-ß-glucanase (EI) and exo-ß-glucanase (ExoII), of which optimum pH are about 5, and molecular distribution of hemicellulosic polysaccharides in barley (Hordeum vulgare L.) coleoptile segments treated with or without IAA. IAA (10–5 M) stimulated the gene expression of EI, while it did not affect that of ExoII. IAA induced gene expression of EI after 4 h and increased wall-bound glucanase activity after 8 h. The molecular weight distribution of hemicellulosic polysaccharides from coleoptile cell walls was shifted to lower molecular weight region by 2 h of IAA treatment. Fusicoccin (10–6 M) mimicked IAA-induced elongation growth and the decrease in molecular weight of hemicellulosic 1,3:1,4-ß-glucan of coleoptiles in the first 4 h, but it did not promote elongation growth thereafter. These facts suggest that acidification of barley cell walls by IAA action enhances pre-existing cell wall-bound glucanase activity in the early first phase of IAA-induced growth and the late second phase involves the gene expression of EI by IAA.

3 Corresponding author: E-mail, nsakura@hiroshima-u.ac.jp; Fax, +81-824-24-0758.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 ANN BOT (LOND)Home page
T. Kimpara, T. Aohara, K. Soga, K. Wakabayashi, T. Hoson, Y. Tsumuraya, and T. Kotake
{beta}-1,3 : 1,4-Glucan Synthase Activity in Rice Seedlings under Water
Ann. Bot., August 1, 2008; 102(2): 221 - 226.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
K. Yoshida and K. Komae
A Rice Family 9 Glycoside Hydrolase Isozyme with Broad Substrate Specificity for Hemicelluloses in Type II Cell Walls
Plant Cell Physiol., November 1, 2006; 47(11): 1541 - 1554.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
J. Wasaki, T. Shinano, K. Onishi, R. Yonetani, J. Yazaki, F. Fujii, K. Shimbo, M. Ishikawa, Z. Shimatani, Y. Nagata, et al.
Transcriptomic analysis indicates putative metabolic changes caused by manipulation of phosphorus availability in rice leaves
J. Exp. Bot., June 1, 2006; 57(9): 2049 - 2059.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
A. J. BLOOM, J. FRENSCH, and A. R. TAYLOR
Influence of Inorganic Nitrogen and pH on the Elongation of Maize Seminal Roots
Ann. Bot., May 1, 2006; 97(5): 867 - 873.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
H. P. d. Santos, E. Purgatto, H. Mercier, and M. S. Buckeridge
The Control of Storage Xyloglucan Mobilization in Cotyledons of Hymenaea courbaril
Plant Physiology, May 1, 2004; 135(1): 287 - 299.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. Roulin and U. Feller
Reversible accumulation of (1->3,1->4)-{beta}-glucan endohydrolase in wheat leaves under sugar depletion
J. Exp. Bot., December 1, 2001; 52(365): 2323 - 2332.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.