Plant and Cell Physiology, 2000, Vol. 41, No. 11 1229-1234
© 2000 Oxford University Press
Stress Tolerance in Transgenic Tobacco Seedlings that Overexpress Glutathione S-Transferase/Glutathione Peroxidase
1 Department of Biological Sciences, Texas Tech University, Lubbock, TX 79409, U.S.A. 2 Department of Plant and Soil Sciences, Texas Tech University, Lubbock, TX 79409, U.S.A. 3 USDA-ARS Cropping Systems Research Laboratory, 3810 4th Street, Lubbock, TX 79424, U.S.A.
Overexpression of a tobacco glutathione S-transferase with glutathione peroxidase activity (GST/GPX) in transgenic tobacco (Nicotiana tabacum L.) enhanced seedling growth under a variety of stressful conditions. In addition to increased GST and GPX activity, transgenic GST/GPX-expressing (GST+) seedlings had elevated levels of monodehydroascorbate reductase activity. GST+ seedlings also contained higher levels of glutathione and ascorbate than wild-type seedlings and the glutathione pools were more oxidized. Thermal or salt-stress treatments that inhibited the growth of wild-type seedlings also caused increased levels of lipid peroxidation. These treatments had less effect on the growth of GST+ seedling growth and did not lead to increased lipid peroxidation. Stress-induced damage resulted in reduced metabolic activity in wild-type seedlings while GST+ seedlings maintained metabolic activity levels comparable to seedlings grown under control conditions. These results indicate that overexpression of GST/GPX in transgenic tobacco seedlings provides increased glutathione-dependent peroxide scavenging and alterations in glutathione and ascorbate metabolism that lead to reduced oxidative damage. We conclude that this protective effect is primarily responsible for the ability of GST+ seedlings to maintain growth under stressful conditions.
4 Present address: Department of Toxinology, USAMRIID, 1425 Porter Street, Frederick, MD 21702, U.S.A.
5 Corresponding author: E-mail, randy.allen@ttu.edu; Fax, +1-806-742-2963; Phone, +1-806-742-2711.
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