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Plant and Cell Physiology, 2000, Vol. 41, No. 10 1171-1174
© 2000 Oxford University Press

Brassinolide and [26, 28-2H6]Brassinolide Are Differently Demethylated by Loss of C-26 and C-28, Respectively, in Marchantia polymorpha

Tae-Wuk Kim1, Soo Chul Chang1, Jongkil Choo1, Tsuyoshi Watanabe2, Suguru Takatsuto3, Takao Yokota4, June Seung Lee5, Soon Young Kim6 and Seong-Ki Kim1,7

1 Department of Life Science, Chung-Ang University, Seoul 156-756, Korea 2 Tama Biochemical Co. Ltd., 2-7-1, Nishishinjuku, Shinjuku-ku, Tokyo, 163-0704 Japan 3 Department of Chemistry, Joetsu University of Education, Joetsu-shi, Niigata, 943-8512 Japan 4 Department of Biosciences, Teikyo University, Utsunomiya, 320-8551 Japan 5 Department of Biological Science, Ewha Womans University, Seoul 120-750, Korea 6 Department of Biology, Andong National University, Andong 760-749, Korea

Metabolism of brassinolide in Marchantia polymorpha was investigated by use of in vivo suspension cultured cells. GC-MS analysis of metabolites derived from non-labelled brassinolide and [26, 28-2H6]brassinolide revealed that brassinolide was converted to 26-norbrassinolide while [26, 28-2H6]brassinolide to [26-2H3]28-norbrassinolide. It seems that Marchantia cells recognized [26, 28-2H6]brassinolide as a xenobiotic rather than brassinolide and deteriums attached to C-28 significantly affect demethylation reaction due to isotopic effect. Thus, demethylation of brassinolide in planta seems to proceed by loss of C-26 rather than C-28. The present finding is the first evidence for demethylation metabolism of brassinosteroids. The biological activity of 26-norbrassinolide was 10-fold reduced as shown by the rice lamina inclination test. However, because of its high biological activity, it remains difficult to conclude whether or not C-26 demethylation serves as an important deactivation process of brassinolide.

7 Corresponding author: E-mail, skkimbio@cau.ac.kr; Fax, +82-2-820-5206.


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