Plant and Cell Physiology, 2000, Vol. 41, No. 10 1157-1163
© 2000 Oxford University Press
Mitochondrial Localization of AtOXA1, an Arabidopsis Homologue of Yeast Oxa1p Involved in the Insertion and Assembly of Protein Complexes in Mitochondrial Inner Membrane
1 Research Institute for Bioresources, Okayama University, Kurashiki, Okayama, 710-0046 Japan 2 Institut de Biologie Moléculaire des Plantes, CNRS, Strasbourg, F-67084 Cedex, France 3 Current address: Nutrition and Toxicology, 119 Morgan Hall, University of California, Berkeley, CA, U.S.A.
Components of some protein complexes present in the inner membrane of mitochondria are encoded in both nuclear and mitochondrial genomes, and correct sorting and assembly of these proteins is necessary for proper respiratory function. Recent studies in yeast suggest that Oxa1p, a protein conserved between prokaryotes and eukaryotes, is an essential factor for protein sorting and assembly into membranes. We previously identified AtOXA1, an Arabidopsis homologue of OXA1 by functional complementation of a yeast oxa1– mutant. In this study, we investigated the genomic organization of AtOXA1 and localization of the AtOXA1 protein. Characterization of the AtOXA1 genomic region indicated that the gene consists of 10 exons and is located on chromosome V. A database search also revealed another gene coding for a putative protein homologous to AtOXA1 on chromosome II. Transient expression of a green fluorescent protein (GFP) fusion in suspension-cultured tobacco cells showed that AtOXA1 is targeted into mitochondria by its N-terminal presequence. Antibodies raised against AtOXA1 recognized a 38-kDa intrinsic protein of the inner mitochondrial membrane. Thus, localization of AtOXA1 in the mitochondrial inner membrane, together with our previous complementation experiment in yeast, suggested that it is a functional homologue of Oxa1p.
4 Corresponding author: E-mail, saka@rib.okayama-u.ac.jp; Fax, +81-86-434-1208.
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