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Plant and Cell Physiology, 2000, Vol. 41, No. 10 1143-1148
© 2000 Oxford University Press

Synthesis of Phosphatidylserine in Carrot Cells Cultured under Carbon-Source Starvation

Kumariah Manoharan1, Hyun Sook Chae1, Jae Myoung1, Sung Ho Cho2, Sung Ho Shin3, Bong Heuy Cho4 and Woo Sung Lee1,5

1 Department of Biology and the Basic Science Research Institute, Sungkyunkwan University, Suwon, 440-746 Korea 2 Department of Biology, Inha University, Inchon, 402-751 Korea 3 CoreBio Inc., Suwon, 445-743 Korea 4 Department of Biology, Suwon University, Suwon, 445-743 Korea

When carrot suspension cells were cultured on medium containing no carbon source (starvation), the levels of phosphatidylserine (PS) increased transiently 3–4 d after the initiation of starvation while levels of most other phospholipid (PL) species decreased. We previously reported that fatty acids of these PLs served as an alternative carbon source during starvation. The present study showed that cells possess two different biosynthetic pathways involving phosphatidylcholine (PC)/phosphatidylethanolamine (PE) exchange enzymes and PS synthase to synthesize PS. These activities peaked similarly 4 d after the initiation of starvation and coincided with the peak of PS level. The synthesis of serine was also significantly activated during starvation. The activity of phosphoserine aminotransferase (PSAT) which is involved in serine synthesis increased with a time course similar to that of the increase in the PS level. These observations suggest that the increase in PS level plays an important role in membranes which are degraded during starvation.

5 Corresponding author: E-mail, wslee@yurim.skku.ac.kr; Fax, +82-31-290-7015.


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