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Plant and Cell Physiology, 2000, Vol. 41, No. 10 1136-1142
© 2000 Oxford University Press

Integrin-Like Proteins in the Pollen Tube: Detection, Localization and Function

Ying Sun1, Hong Qian2, Xiao-dong Xu1, Ye Han1, Long-fei Yen3 and Da-ye Sun1,4

1 Institute of Molecular Cell Biology, Hebei Normal University, Shijiazhuang, Hebei 050016, People’s Republic of China 2 Institute of Vegetables and Flowers, Chinese Academy of Agricultural Science, Beijing 100081, People’s Republic of China 3 Department of Biochemical and Molecular Biology, China Agricultural University, Beijing 100094, People’s Republic of China

The distribution of integrin-like proteins in the pollen tube was examined by immunofluorescent labeling and western blotting techniques using antibodies against human placenta integrin vitronectin receptor (VnR), and {alpha}V, ß3 and ß1 integrin subunits. Pseudocolor-coded confocal images showed intense immunostaining within 10 and 5 µm of the tip of the pollen tube in Lilium davidii and Nicotiana tabacum respectively. In both segments the site near the plasma membrane was labeled. Western blotting analyses revealed cross-reaction of anti-ß3, anti-{alpha}V and anti-VnR with the proteins in the plasma membrane preparation of L. davidii and Hemerocallis citrina pollen tube. These studies provide evidence for the first time that the integrin-like protein is present in pollen tubes, and it may be mainly composed of {alpha}V and ß3 subunits in lily pollen tubes. In a functional assay, neither anti-VnR antibody nor the Arg-Gly-Asp-Ser tetrapeptide inhibited pollen tube growth of N. tabacum in vitro, but both of them depressed tube growth on the stigma and in style under quasi in vivo culture conditions. The integrin-like proteins localized in the tip and periphery of the pollen tube appeared to play roles in growth of the pollen tube tip and interaction with the extracellular matrix of the style.

4 Corresponding author: E-mail, sundaye@public.sj.he.cn; Fax, +86-311-5820649.


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