Plant and Cell Physiology, 1999, Vol. 40, No. 5 489-495
© 1999
Organic Acid Metabolism in Aluminum-Phosphate Utilizing Cells of Carrot (Daucus carota L.)
Laboratory of Plant Cell Technology, Faculty of Agriculture, Gifu University 1-1 Yanagido, Gifu, 501-1193 Japan
1e-mail, koyama{at}cc.gifu-u.ac.jp
Carbohydrate metabolism in Al-phosphate utilizing cells of carrot [designated as IPG, Koyama et al. (1992) Plant Cell Physiol. 33: 171], which grow normally in Al-phosphate medium accompanied by citrate excretion, was investigated. The excretion of citrate was strongly related to the availability of sucrose in medium, indicating that citrate excretion was severely limited by sucrose in medium. The ratio of the amount of carbon in the excreted citrate to the consumed sucrose, was significantly higher in IPG cells than in wild-type cells. When 50% of the sucrose in the medium was consumed, the ratio was 0.6% for the IPG cells and 0.2% the wild-type cells. Under these conditions, IPG cells showed altered citrate synthesis metabolism, which resulted in increased citrate production. Specific activity of mitochondrial citrate synthase was higher in IPG cells than in wild-type cells, whereas the activity of cytosolic NADP-specific isocitrate dehydrogenase was lower in IPG cells than in wild-type cells.
(Received August 27, 1998; Accepted February 21, 1999)
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  Y. Kobayashi, O. A. Hoekenga, H. Itoh, M. Nakashima, S. Saito, J. E. Shaff, L. G. Maron, M. A. Pineros, L. V. Kochian, and H. Koyama Characterization of AtALMT1 Expression in Aluminum-Inducible Malate Release and Its Role for Rhizotoxic Stress Tolerance in Arabidopsis Plant Physiology, November 1, 2007; 145(3): 843 - 852. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Iuchi, H. Koyama, A. Iuchi, Y. Kobayashi, S. Kitabayashi, Y. Kobayashi, T. Ikka, T. Hirayama, K. Shinozaki, and M. Kobayashi Zinc finger protein STOP1 is critical for proton tolerance in Arabidopsis and coregulates a key gene in aluminum tolerance PNAS, June 5, 2007; 104(23): 9900 - 9905. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Kihara, T. Wada, Y. Suzuki, T. Hara, and H. Koyama Alteration of Citrate Metabolism in Cluster Roots of White Lupin Plant Cell Physiol., September 15, 2003; 44(9): 901 - 908. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. M. Anoop, U. Basu, M. T. McCammon, L. McAlister-Henn, and G. J. Taylor Modulation of Citrate Metabolism Alters Aluminum Tolerance in Yeast and Transgenic Canola Overexpressing a Mitochondrial Citrate Synthase Plant Physiology, August 1, 2003; 132(4): 2205 - 2217. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Ohno, H. Koyama, and T. Hara Characterization of Citrate Transport through the Plasma Membrane in a Carrot Mutant Cell Line with Enhanced Citrate Excretion Plant Cell Physiol., February 15, 2003; 44(2): 156 - 162. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Kobayashi and H. Koyama QTL Analysis of Al Tolerance in Recombinant Inbred Lines of Arabidopsis thaliana Plant Cell Physiol., December 15, 2002; 43(12): 1526 - 1533. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Aono, N. Kanada, A. Ijima, and H. Oyaizu The Response of the Phosphate Uptake System and the Organic Acid Exudation System to Phosphate Starvation in Sesbania rostrata Plant Cell Physiol., October 1, 2001; 42(11): 1253 - 1264. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Delhaize, D. M. Hebb, and P. R. Ryan Expression of a Pseudomonas aeruginosa Citrate Synthase Gene in Tobacco Is Not Associated with Either Enhanced Citrate Accumulation or Efflux Plant Physiology, April 1, 2001; 125(4): 2059 - 2067. [Abstract] [Full Text]
|
|
|
|
|
|
H. Koyama, A. Kawamura, T. Kihara, T. Hara, E. Takita, and D. Shibata Overexpression of Mitochondrial Citrate Synthase in Arabidopsis thaliana Improved Growth on a Phosphorus-Limited Soil Plant Cell Physiol., September 1, 2000; 41(9): 1030 - 1037. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. F. Li, J. F. Ma, and H. Matsumoto Pattern of Aluminum-Induced Secretion of Organic Acids Differs between Rye and Wheat Plant Physiology, August 1, 2000; 123(4): 1537 - 1544. [Abstract] [Full Text]
|
|