Plant and Cell Physiology, 1999, Vol. 40, No. 4 378-387
© 1999
Sequence analysis of Expressed Sequence Tags from an ABA-Treated cDNA Library Identifies Stress Response Genes in the Moss Physcomitrella patens
Centre for Plant Sciences, Leeds Institute for Plant Biotechnology and Agriculture (LIBA), School of Biology, University of Leeds Leeds, LS2 9JT, U.K.
Partial cDNA sequencing was used to obtain 169 expressed sequence tags (ESTs) in the moss, Physcomitrella patens. The source of ESTs was a random cDNA library constructed from 7 day-old protonemata following treatment with 10–4 M abscisic acid (ABA). Analysis of the ESTs identified 69% with homology to known sequences, 61% of which had significant homology to sequences of plant origin. More importantly, at least 11 ESTs had significant similarities to genes which are implicated in plant stress-responses, including responses which may involve ABA. These included a cDNA associated with desiccation tolerance, two heat shock protein genes, one cold acclimation protein cDNA and five others that may be involved in either oxidative or chemical stress or both, i.e., Zn/Cu-superoxide dismutase, NADPH protochlorophyllide oxidoreductase (PorB), selenium binding protein, glutathione peroxidase and glutathione S transferase. Analysis of codon usage between P. patens and seed plants indicated that although mosses and higher plants are to a large extent similar, minor variations also exist that may represent the distinctiveness of each group.
1 Present address: International Institute of Tropical Agriculture, c/o LW Lambourn & Co. LTD, Carolyn House, 26 Dingwall Road, Croydon CR9 3EE, U.K.
(Received September 10, 1998; Accepted January 30, 1999)
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  C. Sugiura, Y. Kobayashi, S. Aoki, C. Sugita, and M. Sugita Complete chloroplast DNA sequence of the moss Physcomitrella patens: evidence for the loss and relocation of rpoA from the chloroplast to the nucleus Nucleic Acids Res., September 15, 2003; 31(18): 5324 - 5331. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Nishiyama, T. Fujita, T. Shin-I, M. Seki, H. Nishide, I. Uchiyama, A. Kamiya, P. Carninci, Y. Hayashizaki, K. Shinozaki, et al. Comparative genomics of Physcomitrella patens gametophytic transcriptome and Arabidopsis thaliana: Implication for land plant evolution PNAS, June 24, 2003; 100(13): 8007 - 8012. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Breton, J. Danyluk, J.-B. F. Charron, and F. Sarhan Expression Profiling and Bioinformatic Analyses of a Novel Stress-Regulated Multispanning Transmembrane Protein Family from Cereals and Arabidopsis Plant Physiology, May 1, 2003; 132(1): 64 - 74. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Liu and Wm. V. Baird Differential Expression of Genes Regulated in Response to Drought or Salinity Stress in Sunflower Crop Sci., March 1, 2003; 43(2): 678 - 687. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Chen, T. Kanokporn, Q. Zeng, T. A. Wilkins, and A. J. Wood Characterization of the V-type H(+)-ATPase in the resurrection plant Tortula ruralis: accumulation and polysomal recruitment of the proteolipid c subunit in response to salt-stress J. Exp. Bot., February 1, 2002; 53(367): 225 - 232. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y Sato, M Wada, and A Kadota Choice of tracks, microtubules and/or actin filaments for chloroplast photo-movement is differentially controlled by phytochrome and a blue light receptor J. Cell Sci., January 1, 2001; 114(2): 269 - 279. [Abstract] [PDF]
|
|
|
|
|
|
M. C.F. Proctor and N. Smirnoff Rapid recovery of photosystems on rewetting desiccation-tolerant mosses: chlorophyll fluorescence and inhibitor experiments J. Exp. Bot., October 1, 2000; 51(351): 1695 - 1704. [Abstract] [Full Text] [PDF]
|
|