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Plant and Cell Physiology, 1999, Vol. 40, No. 2 231-237
© 1999

Auxin Polar Transport and Flower Formation in Arabidopsis thaliana Transformed with Indoleacetamide Hydrolase (iaaH) Gene

Mariko Okal1,3, Kensuke Miyamoto1, Kiyotaka Okada2 and Junichi Ueda1,4

1 College of Integrated Arts and Sciences, Osaka Prefecture University 1-1 Gakuen-cho, Sakai, Osaka, 599-8531 Japan
2 Faculty of Science, Kyoto University Kyoto, 606-8502 Japan

4 To whom all correspondence should be addressed

Involvement of auxin polar transport in flower formation of Arabidopsis thaliana was studied using a pinformed (pin) mutant (Rpin) transformed with the indole-acetamide hydrolase (iaaH) gene and the phenocopy of the pin mutant, which was induced by 9-hydroxyfluorene-9- carboxylic acid (HFCA). The application of indoleacetamide (IAM) did not change aberrant structure of the aerial part of Rpin (pin/pin), but extremely inhibited its root growth. Treatment with IAM increased the endogenous concentrations of free and conjugated IAA in Rpin normal (pin/+ or +/+) due to the expression of the iaaH gene, to 140% and 428% of those in non-treated plants, respectively, and those in Rpin to 378% and 120%, respectively. The activity of IAA polar transport in the inflorescence axis of Rpin remained low even in the presence of IAM, the activity being almost similar, to that in the pin mutant. The activity of IAA polar transport in the HFCA-induced phenocopy of the pin mutant was also extremely low, and it was not restored by the simultaneous application of IAA. Arabidopsis thaliana responded to HFCA applied from 7 to 11 d and from 25 to 29 d after germination in the wild-type plant (Enkheim ecotype) and the late flowering mutant (fb mutant), respectively. These results suggest that the construction of the system of auxin polar transport and its normal activities are essential for the differentiation and the formation of floral meristem in the early growth stage of Arabidopsis thaliana.

3 Present address: Research Institute for Biological Sciences Okayama, 7549-1 Yoshikawa, Kayo-cho, Joubo-gun, Okayama, 716-1241 Japan.

(Received May 22, 1998; Accepted December 8, 1998)
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