Plant and Cell Physiology, 1999, Vol. 40, No. 2 222-230
© 1999
Function of Ngrol Genes in the Evolution of Nicotiana glauca: Conservation of the Function of NgORF13 and NgORF14 after Ancient Infection by an Agrobacterium rhizogenes-Like Ancestor
Aoki1,no2Department of Life Science, Graduate School of Arts and Sciences, University of Tokyo 3-8-1 Komaba, Meguro-ku, Tokyo, 153-8902 Japan
1 To whom correspondence should be addressed
Ngrol genes are thought to have resulted from horizontal gene transfer from an Agrobacterium rhizogenes-like ancestor early in the evolution of the genus Nicotiana. Four Ngrol genes (NgrolB, NgrolC, NgORF13 and NgORF14) have been found in the genome of N. glauca, but their functions are not yet known. We have investigated the properties of Ngrol genes and shown that some of them are able to function in tobacco plants. Transgenic analysis revealed that NgORF13 promotes RirolB-mediated adventitious root induction on tobacco leaf segments. NgORF14 also promoted the RirolB-mediated root induction, but the intensity of this promoting effect was weak. These promoting functions of NgORF13 and NgORF14 have much the same efficiency as those of the corresponding genes of A. rhizogenes, RiORF13 and RiORF14, respectively. Overexpression of NgORF13, under control of the cauliflower mosaic virus 35S promoter (P35s), provoked morphological abnormalities in transgenic tobacco plants. Transgenic plants that harbored the P35s-NgORF13 had rounded leaves and stout flowers resulting from suppression of the longitudinal growth of leaf and floral leaves such as sepals, petals, stamens and carpels. These results suggest that NgORF13 and NgORF14 in the genome of N. glauca have conserved functional sequences since their original integration event by an A. rhizogenes-like ancestor.
Present address: Laboratory of Phylogenetic Botany, Department of Biology, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba, 263-8522 Japan.
2 Present address: Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University, 2-8-1 Mejirodai, Bunkyo-ku, Tokyo, 112-8681 Japan.
(Received June 5, 1998; Accepted December 6, 1998)
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