Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Nishiyama, R.
Right arrow Articles by Ohyama, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nishiyama, R.
Right arrow Articles by Ohyama, K.
Agricola
Right arrow Articles by Nishiyama, R.
Right arrow Articles by Ohyama, K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 1999, Vol. 40, No. 2 205-212
© 1999

Two mRNA Species Encoding Calcium-Dependent Protein Kinases Are Differentially Expressed in Sexual Organs of Marchantia polymorpha through Alternative Splicing

Rie Nishiyama, Hiroshi Mizuno, Sachiko Okada, Tomoya Yamaguchi, Mizuki Takenaka, Hideya Fukuzawa and Kanji Ohyama1

Laboratory of Plant Molecular Biology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University Kyoto, 606-8502 Japan

1 To whom correspondence should be addressed. Phone, +81-75-753-6389; FAX, +81-75-753-6127; E-mail, kohyama{at}kais.kyoto-u.ac.jp

In plants, calcium-dependent calmodulin-independent protein kinases (CDPKs) are the predominant calcium- regulated protein kinases and their genes are encoded by a multigene family. A CDPK gene was cloned from a liverwort, Marchantia polymorpha, which showed a high level of sequence similarities to other higher plant CDPK genes. The liverwort CDPK gene consisted of 9 exons and 8 introns. The 6th and 7th exons (Exon 6A and Exon 6B) were almost identical except for 4-amino acid substitutions, both of which coded for EF-hands in the calciumbinding domain. RT-PCR analysis revealed that two species of mature mRNA containing either Exon 6A or Exon 6B were generated from a single CDPK gene by mutually exclusive alternative splicing. Both histidine-tagged fusion proteins derived from cDNAs containing either Exon 6A or Exon 6B exhibited calcium-dependent protein kinase activity in vitro. Preferential accumulation of the mature mRNA with Exon 6A detected in male sexual organ implies possible sexual control of the ratio between the two CDPK isozymes through alternative splicing. Functions and evolution of CDPKs are discussed based on the structure and expression of the liverwort CDPK gene.

(Received October 28, 1998; Accepted December 2, 1998)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Cell PhysiolHome page
T. Asano, N. Tanaka, G. Yang, N. Hayashi, and S. Komatsu
Genome-wide Identification of the Rice Calcium-dependent Protein Kinase and its Closely Related Kinase Gene Families: Comprehensive Analysis of the CDPKs Gene Family in Rice
Plant Cell Physiol., February 1, 2005; 46(2): 356 - 366.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S.-H. Cheng, M. R. Willmann, H.-C. Chen, and J. Sheen
Calcium Signaling through Protein Kinases. The Arabidopsis Calcium-Dependent Protein Kinase Gene Family
Plant Physiology, June 1, 2002; 129(2): 469 - 485.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
M. Fujisawa, K. Hayashi, T. Nishio, T. Bando, S. Okada, K. T. Yamato, H. Fukuzawa, and K. Ohyama
Isolation of X and Y Chromosome-Specific DNA Markers From a Liverwort, Marchantia polymorpha, by Representational Difference Analysis
Genetics, November 1, 2001; 159(3): 981 - 985.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Okada, T. Sone, M. Fujisawa, S. Nakayama, M. Takenaka, K. Ishizaki, K. Kono, Y. Shimizu-Ueda, T. Hanajiri, K. T. Yamato, et al.
The Y chromosome in the liverwort Marchantia polymorpha has accumulated unique repeat sequences harboring a male-specific gene
PNAS, July 31, 2001; 98(16): 9454 - 9459.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Tassoni, R. M. Napier, M. Franceschetti, M. A. Venis, and N. Bagni
Spermidine-Binding Proteins. Purification and Expression Analysis in Maize
Plant Physiology, April 1, 2002; 128(4): 1303 - 1312.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.