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Plant and Cell Physiology, 1999, Vol. 40, No. 2 198-204
© 1999

Identification and cDNA Cloning of a Protein Abundantly Expressed during Apple Fruit Development

Kunio Yamada, Hitoshi Mori and Shohei Yamaki1

Graduate School of Bioagricultural Sciences, Nagoya University Chikusa, Nagoya, 464-8601 Japan

1 fax +81-52-789-4025

A 60 kDa protein (MF-60) abundantly appearing in matured apple fruit was detected by SDS-PAGE of the soluble protein. It was partially purified through Butyl-Toyopearl and DEAE-cellulose. Its partial ami no acid sequences were determined to isolate a full-length cDNA. MF-60 cDNA (mf-60) consisting of 1,825 bp containing an open reading frame of 1,524 bp and encoding a 54.2 kDa polypeptide. The deduced polypeptide of mf-60 has 81.1% identity to turgor-responsive protein 26 g from wilted garden pea shoot. Northern blot and Western blot analyses showed that the levels of the protein and the transcript of MF-60 changed in parallel through the developmental season; they were very low in young fruit at 36 DAF and 60 DAF, started to increase at 85 DAF, and then remained at a higher level from 114 DAF to 176 DAF. These results suggested that MF-60 functions are connected with fruit development but not with the fruit ripening induced by ethylene.

(Received September 20, 1998; Accepted December 2, 1998)
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