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Plant and Cell Physiology, 1999, Vol. 40, No. 2 184-191
© 1999

Abscisic Acid-Induced Secretory Proteins in Suspension-Cultured Cells of Winter Wheat

Chikako Kuwabara, Keita Arakawa1 and Shizuo Yoshida

Institute of Low Temperature Science, Hokkaido University Sapporo, 060-0819 Japan

1 To whom correspondence should be addressed

In suspension-cultured cells of winter wheat (Triticum aestivum L. cv. Chihokukomugi), the accumulation of soluble secretory proteins in the culture medium was promoted by ABA treatment in comparison with non-treated cells. The total amount of secretory proteins in ABA-treated cells was 1.7-fold higher than that in non-treated cells. The analysis of two-dimensional electrophoresis revealed that at least twelve secretory proteins were induced by ABA, and these were named WAS (wheat ABA-induced secretory) proteins 1 to 12. N-terminal amino acid sequence analysis of WAS proteins revealed the sequences of WAS-2 and WAS-3. Homology searches showed that WAS-2 had 55% identity with the N-terminus of the wheat chemically induced gene (WCI-5 gene) product. WAS-3 was also shown to have 93% identity with the N-terminus of the barley protein R, a typical member of thaumatin-like proteins (TLPs). Immunoblot analysis also suggested that WAS-3 was related to protein R. These results suggest that exogenous ABA induces some basic secretory proteins that are related to the plant defense system in wheat.

(Received August 20, 1998; Accepted November 27, 1998)
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