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Plant and Cell Physiology, 1999, Vol. 40, No. 2 149-154
© 1999

Chloroplast NADH Dehydrogenase from Pisum sativum: Characterization of its Activity and Cloning of ndhK Gene

Felix Elortza, Juan A. Asturias1 and Jesús M. Arizmendi2

Biokimika eta Biologia Molekularreko Saila, Euskal Herriko Unibertsitatea 644 Posta kutxatila. 48080 Bilbao. Spain

2 fax, 34 94 4648500; e-mail address, gbparbaj{at}lg.ehu.es

The pea chloroplast ndhK gene coding for a component of a NADH-plastoquinone oxidoreductase has been cloned and sequenced. This gene codes for a polypeptide of 227 amino acids and a predicted molecular mass of 25,495 Da which belongs to the family of the 20 kDa PSST subunit of the bovine mitochondrial complex I. A fragment of this gene has been overexpressed in Escherichia coli, and antibodies against the expressed polypeptide recognize a protein of the predicted molecular mass from pea thylakoid membranes. This polypeptide is a component of a protein complex with NADH dehydrogenase activity and is not associated with ferredoxin-NADP+reductase.

1 Present address: IFIDESA-Aristegi laborategia. Alameda de Urkijo 27. 48008 Bilbao. Spain.

(Received June 29, 1998; Accepted November 18, 1998)
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