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Plant and Cell Physiology, 1998, Vol. 39, No. 6 632-638
© 1998

Article

Cell Wall Architecture Prerequisite for the Cell Division in the Protoplasts of White Poplar, Populus alba L.

Kiyoshi Suzuki1, Takao Itoh1 and Hamako Sasamoto2

1 Wood Research Institute, Kyoto University Uji, Kyoto, 611 Japan
2 Forestry and Forest Products Research Institute Tsukuba, Ibaraki, 305 Japan

White poplar (Populus alba L.) protoplasts were investigated at 0, 3,10, 20 and 30 d after regeneration to visualize the cell wall architecture prerequisite for cell division. The 10 day-old cells just before cell division developed a thin wall layer with uneven deposition of cell wall materials and were saved from bursting by suspension in low osmotic medium. The three dimensional architecture of the cell wall, as revealed by rapid-freezing and deep-etching electron microscopy, in 10 day-old cells, constituted thin innermost lamellae on the plasma membrane along with highly extended micronbrillar networks. These results suggest that the deposition of thin lamellae is important not only for cells to withstand bursting but also to induce cell division. The present investigations give the first account of the visualization of the three-dimensional architecture of regenerated cell wall right before cell division.

(Received July 31, 1997; Accepted April 6, 1998)
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