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Plant and Cell Physiology, 1997, Vol. 38, No. 9 985-999
© 1997

Interrelationships between Cytoplasmic Ca2+ Peaks, Pollen Hydration and Plasma Membrane Conductances during Compatible and Incompatible Pollinations of Brassica napus Papillae

John D.W. Dearnaley1, Natalia N. Levina2, Roger R. Lew1, I. Brent Heath1,3 and Daphne R. Goring1

1 Department of Biology, York University 4700 Keele Street, North York, Ontario M3J1P3, Canada

3Author for correspondence (Fax: 416 736 5698, e-mail: brent{at}yorku.ca).

We have investigated Ca2+-involving cell signaling, plasma membrane potentials and conductances and callose formation during early stages of pollination of papillae of Brassica napus. Using fluorescence imaging of calcium green-1, we found that application of a range of pollen types and controls all rapidly produced small localized peaks in papillar cytoplasmic [Ca2+]. This response was more frequent in compatible than incompatible interactions and was correlated with subsequent hydration of the applied pollen grains, indicating that it may be a differential prerequisite of the compatible signaling pathway leading to successful pollinations. In contrast, a slight trend to increased plasma membrane conductance (but with no indications of action potential-like responses) and also callose deposition in papillae adjacent to pollen grains followed pollination in both SC and SI interactions, indicating that alterations in plasma membrane permeability and callose deposition during early phases of pollination are not primary determinants of the fate of attempted pollinations.

2On leave of absence from A.N. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky Pr., 33, Moscow, Russia, 117071

(Received April 24, 1997; Accepted June 13, 1997)
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