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Plant and Cell Physiology, 1997, Vol. 38, No. 3 282-289
© 1997

Cs+ Uptake in Subapical Maize Root Segments: Mechanism and Effects on H+ Release, Transmembrane Electric Potential and Cell pH

Gian Attilio Sacchi, Luca Espen, Fabio Nocito and Maurizio Cocucci

Dipartimento di Fisiologia delle Piante Coltivate e Chimica Agraria (DIFCA), Universita degli Studi di Milano via Celoria, 2, 20133 Milan, Italy

Subapical segments from maize (Zea mays) root took up Cs+; at low external concentrations (less double equals0.25 mM) the kinetic constants for the influx of this cation were similar to those for the uptake of Rb+ and K+ (Km=26, 26 and 22 µM and Vmax = 2.62, 3.94 and 4.09µmol h–1 (gFW)–1 respectively). Competition experiments suggested that the three cations use the same transport system. At higher concentrations (>0.25mM) the discrimination between Cs+ and Rb+ increased. At low external concentrations, the release of H+ into the medium was promoted similarly by Cs+, Rb+ or K+; at higher concentration (5 mM) the H+ release was higher in the presence of K+ than with Rb+ or Cs+. The transmembrane electrical potential difference (Em) was depolarised when Cs+, Rb+ or K+ were present in the incubation media; this depolarisation was greater in the presence of K+ and was particularly evident at higher concentrations: the Em was –85 mV, –105 mV and –119 mV in 5 mM K+, Rb+ or Cs+, respectively. Tetraethylam-monium-chloride, a K+-channel blocker, strongly hyper-polarized Era in the presence of K+ but had little or no effect with Rb+ or Cs+, respectively. The influx of Cs+ was accompanied by less dark fixation of CO2 than was that of K+ or Rb + . These results are consistent with the activity of a system showing low selectivity between Cs+, Rb+ and K+, operating primarily at low external concentrations, which possibly uses a cation-H+ co-uptake mechanism, and of a channel-mediated system more selective for K+, operating at higher concentrations; these systems differ in their involvement of H+ transport activity and the metabolic pH-stat mechanism.

(Received September 4, 1996; Accepted December 17, 1996)
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