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Plant and Cell Physiology, 1997, Vol. 38, No. 3 268-273
© 1997

Conversion of Indole-3-Acetaldehyde to Indole-3-Acetic Acid in Cell-Wall Fraction of Barley (Hordeum vulgare) Seedlings

Ken-ichi Tsurusaki1, Kazuyoshi Takeda2 and Naoki Sakurai3

1 Faculty of Liberal Arts, Fukuyama University Fukuyama, 729-02 Japan
2 Research Institute for Bioresources, Okayama University Kurashiki, Okayama, 710 Japan
3 Department of Environmental Studies, Faculty of Integrated Arts & Sciences, Hiroshima University Higashi-Hiroshima, 739 Japan

The cell-wall fraction of barley seedlings was able to oxidize indole-3-acetaldehyde (IAAld) to form IAA, whereas the fraction did not catalyze the conversion of in-dole-3-acetonitrile or indole-3-acetamide to IAA. The activity was lower in a semi-dwarf mutant that had an endogenous IAA level lower than that of the normal isogenic strain [Inouhe et al. (1982) Plant Cell Physiol. 23: 689]. The soluble fraction also contained some activity; the activity was similar in the normal and mutant strains. The optimal pH for the conversion of IAAld to IAA in the cell-wall fraction was 7; that of soluble fraction was 6. The Km value of the cell-wall fraction for IAAld was 5 µM; that of soluble fraction was 31 µM. The activity was not solubi-lized by treatments with 1% Nonidet P-40,1 M NaCI, 3 M LiCl, or 50 mM MgCl2. The oxidation activity was increased by the addition of NAD+. These results suggest that IAAld oxidation activity is bound to cell-wall components and that the lower level of IAA in the mutant probably results from reduced activity of oxidation enzyme bound to cell-wall components.

(Received July 31, 1996; Accepted December 16, 1996)
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