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Plant and Cell Physiology, 1997, Vol. 38, No. 2 149-154
© 1997

Expression of a Gene for Uricase II (Nodulin-35) in Cotyledons of Soybean Plants

Kenichi Takane1, Koji Tanaka2, Shigeyuki Tajima1, Katsuichiro Okazaki1 and Hiroshi Kouchi3

1 Dept. of Bio resource Science, Faculty of Agriculture, Kagawa University Ikenobe, Miki-cho, Kita-gun, Kagawa, 761-07 Japan
2 Research & Development Center, Maruho Corporation Takamiya 2763, Hikone City, Shiga, 522-02 Japan
3 Dept. of Applied Physiology, National Institute of Agrobiological Resources Tsukuba City, Ibaraki, 305 Japan

A cDNA clone (URcot-35) was isolated from a soybean cotyledonary cDNA library using a cDNA clone (UR-nod-35) for nodule uricase II as a probe. URcot-35 was a 1,170-bp cDNA with an open reading frame that encoded a protein of putative 309 amino acids with a molecular mass of 35,137 Da. The nucleotide sequence of URcot-35 was identical to that of URnod-35.

Expression of the URcot-35 gene in cotyledons was investigated by Northern dot-blot hybridization, by the reverse transcription-polymerase chain reaction with subsequent hybridization assays with the cDNA for nodule uricase II as a probe, and by immunoblotting analysis with a monoclonal antibody that was specific to nodule uricase II. The results suggested that the transcript of URcot-35 was present in developing cotyledons and that uricase II accumulated during the pod-filling stage. This is the first report of the isolation of cDNA for uricase II from non-symbiotic tissue and the results demonstrate that uricase II in soybean cotyledons is identical to that in soybean nodules.

(Received June 24, 1996; Accepted November 23, 1996)
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