Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Nam, K. H.
Right arrow Articles by Lee, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nam, K. H.
Right arrow Articles by Lee, J.
Agricola
Right arrow Articles by Nam, K. H.
Right arrow Articles by Lee, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 1997, Vol. 38, No. 10 1150-1155
© 1997

Purification and Characterization of Arginine Decarboxylase from Soybean (Glycine max) Hypocotyls

Kyoung Hee Nam, Sun Hi Lee and JooHun Lee1

Department of Biology, Yonsei University Seoul, Korea 120-749

1 To whom correspondence should be addressed.

Arginine decarboxylase (EC 4.1.1.19 [EC] ) was purified from soybean, Glycine max, hypocotyls by a procedure which includes ammonium sulfate fractionation, acetone precipitation, gel filtration chromatography, and affinity chromatography. Using this procedure, ADC was purified to one band in non-denaturing PAGE. The purified ADC has an Mr of 240 kDa based on gel filtration chromatography and is a trimer of identical subunits which has an estimated Mr of 74 kDa based on SDS-PAGE. ADC is active between 30 and 50°C and has a Km value of 46.1 µM. ADC is very sensitive to agmatine or putrescine but not to spermidine or spermine. In the presence of 0.5 mM agmatine (or putrescine), the enzyme activity was inhibited by 70%. However, at the same concentration of spermidine (or spermine), the enzyme activity was inhibited by only 10–20%.

(Received April 2, 1997; Accepted August 18, 1997)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.