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Plant and Cell Physiology, 1996, Vol. 37, No. 8 1188-1192
© 1996

Short Communication

Preparation of a Monoclonal Antibody against Soybean Nodule Uricase (nod-35), and Immunoblot Analysis of the Expression of nod-35 in Tissues of Various Legumes

Shigeyuki Tajima, Kenichi Takane, Kyoko Ohkawa, Akio Sugimoto and Katsuichiro Okazaki

Dept. of Bioresource Science, Faculty of Agriculture, Kagawa University Ikenobe, Miki-cho, Kita-gun, Kagawa, 761-07 Japan

Immunoblot analysis showed that uricases in nonureide-transporting determinate nodules (Canavalia gladiata and Lotus japonicus) did not react with a monoclonal antibody against soybean nodule uricase, suggesting different immunological reactivities from those of uricases of ureide-transporting legumes.

(Received June 1, 1996; Accepted September 17, 1996)
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