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Plant and Cell Physiology, 1996, Vol. 37, No. 8 1059-1065
© 1996

Weak Light-Induced Oxygen Consumption Observed during Photoreactivation Is Coupled to the Recovery of Oxygen Evolving Activity

Masumi Ebina1 and Takashi Yamashita2

1National Grass Land Research Institute Senbonmatsu, Nishinasuno, Nasugun, Tochigi, 329-27 Japan
2Institute of Biological Sciences, University of Tsukuba Tsukuba, Ibaraki, 305 Japan

During photoreactivation of the O2-evolving center in Tris-inactivated/Mn-depleted thylakoids, a slow O2-consumption occurred. This O2-consumption became detectable when the O2-evolving activity of thylakoids was inactivated by Tris-treatment and decreased as photoreactivation proceeded. The O2-consumption and photoreactivation similarly required Mn2+ at µM levels in addition to PSII electron donors and shared severa common characteristics. Stimulation of O2-consumption and photoreactivation by these cofactors were always accompanied by enhancement in chlorophyll fluorescence induction, suggesting the involvement of a Mehler-type reaction in photoreactivation. Although the electron transport due to this O2-consumption was rapid enough to oxidize 4 Mn2+ ions to reconstitute the tetranuclear Mn-cluster in each O2-evolving center in a few seconds, actual recovery of O2-evolving activity occurred more slowly in a few minutes. It was inferred that photoreactivation in Tris-inactivated thylakoids is not a simple photooxidation of Mn22+ but involves more complicated processes which are coupled to the Mehlertype electron transport from PSII to oxygen via PSI.

(Received July 11, 1994; Accepted August 23, 1996)
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