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Plant and Cell Physiology, 1996, Vol. 37, No. 3 401-403
© 1996

Short Communication

Suppression by 5-Bromo-2'-deoxyuridine of Transdifferentiation into Tracheary Elements of Isolated Mesophyll Cells of Zinnia elegans

Yuichi Shoji1, Munetaka Sugiyama2 and Atsushi Komamine2,3

1Research & Development Center, Tohoku Electric Power Co. Sendai, 981 Japan
2Biological Institute, Graduate School of Science, Tohoku University Sendai, 980-77 Japan

5-Bromo-2'-deoxyuridine (BrdU), a thymidine analog, suppressed the transdifferentiation into tracheary elements (TE) of isolated mesophyll cells of Zinnia elegans without affecting cell division. Tracer experiments with [3H]BrdU indicated that 76% and 24% of the incorporated radioactivity was located in the DNA and the RNA, respectively. Both thymidine and uridine counteracted the inhibitory effect of BrdU on transdifferentiation but thymidine was much more effective than uridine. These results suggest that BrdU might interfere with transdifferentiation via its incorporation into DNA. The timing of effective suppression by BrdU was examined by monitoring the effects of the sequential addition of BrdU and thymidine with an interval of 12 h at various times in culture. Transdifferentiation was most sensitive to BrdU between the 24th and the 36th hour of culture. This result suggests that this window of time is critical for DNA synthesis during the transdifferentiation of isolated mesophyll cells of Zinnia elegans into TE.

3Present address: Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University, Mejiro, Tokyo, 112 Japan

(Received November 6, 1995; Accepted February 9, 1996)
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