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Plant and Cell Physiology, 1996, Vol. 37, No. 3 385-388
© 1996

Short Communication

A Sodium Pump in the Plasma Membrane of the Marine Alga Heterosigma akashiwo

Mariko Shono1, Yukichi Hara2, Masato Wada3 and Tadashi Fujii3

1Plantech Research Institute 1000 Kamashida-cho, Aoba-ku, Yokohama, 227 Japan
2Department of Biochemistry, Tokyo Medical and Dental University School of Medicine 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113 Japan
3Institutue of Biological Sciences, University of Tsukuba Tsukuba, Ibaraki, 305 Japan

ATP-dependent transport of 22Na+ into liposomes reconstituted from plasma membrane proteins of Heterosigma akashiwo was examined. The apparent Km values for transport of Na+ were 400 µM for ATP and 7 mM for Na+. ATP-dependent transport of 22Na+ was not inhibited by a protonophore or a membrane-permeable cation but was inhibited by an inhibitor of P-type ATPases.

(Received October 2, 1995; Accepted February 1, 1996)
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