Plant and Cell Physiology, 1996, Vol. 37, No. 3 293-298
© 1996
Identification of the Product of ndhA Gene as a Thylakoid Protein Synthesized in Response to Photooxidative Treatment
Departamento de Biología Vegetal, Universidad de Alcalá de Henares, Alcalá de Henares 28871-Madrid, Spain
A 76 amino acid sequence of NDH-A (the protein encoded by plastid ndhA gene) from barley (Hordeum vulgare L.) was expressed as a fusion protein with rß-galactosidase in E. coli. The corresponding antibody generated in rabbits was used to investigate localization, expression and synthesis in vitro of NDH-A. NDH-A was identified as a 35 kDa polypeptide localized in thylakoid membrane. Western blots shows a large increase in NDH-A levels when barley leaves were incubated under photooxidative conditions, which was more pronounced in mature-senescent leaves than in young leaves. Immunoprecipitation of the [35S]methionine labelled proteins, synthesized in vitro by isolated chloroplasts, demonstrated the synthesis in chloroplasts of the NDH-A 35 kDa polypeptide when barley leaves had been incubated under photooxidative conditions. The results indicate that ndh genes may be involved in the protection of chloroplasts against photooxidative stress, particularly in mature-senescent leaves.
(Received November 13, 1995; Accepted February 5, 1996)
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. R. Aluru, F. Yu, A. Fu, and S. Rodermel Arabidopsis variegation mutants: new insights into chloroplast biogenesis J. Exp. Bot., June 1, 2006; 57(9): 1871 - 1881. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Wang, W. Duan, A. Takabayashi, T. Endo, T. Shikanai, J.-Y. Ye, and H. Mi Chloroplastic NAD(P)H Dehydrogenase in Tobacco Leaves Functions in Alleviation of Oxidative Damage Caused by Temperature Stress Plant Physiology, June 1, 2006; 141(2): 465 - 474. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Ifuku, Y. Yamamoto, T.-a. Ono, S. Ishihara, and F. Sato PsbP Protein, But Not PsbQ Protein, Is Essential for the Regulation and Stabilization of Photosystem II in Higher Plants Plant Physiology, November 1, 2005; 139(3): 1175 - 1184. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Guera, A. Calatayud, B. Sabater, and E. Barreno Involvement of the thylakoidal NADH-plastoquinone-oxidoreductase complex in the early responses to ozone exposure of barley (Hordeum vulgare L.) seedlings J. Exp. Bot., January 1, 2005; 56(409): 205 - 218. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. R. Lascano, L. M. Casano, M. Martin, and B. Sabater The Activity of the Chloroplastic Ndh Complex Is Regulated by Phosphorylation of the NDH-F Subunit Plant Physiology, May 1, 2003; 132(1): 256 - 262. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. M. del Campo, B. Sabater, and M. Martin Post-transcriptional Control of Chloroplast Gene Expression. ACCUMULATION OF STABLE psaC mRNA IS DUE TO DOWNSTREAM RNA CLEAVAGES IN THE ndhD GENE J. Biol. Chem., September 20, 2002; 277(39): 36457 - 36464. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. Casano, M. Martín, and B. Sabater Hydrogen Peroxide Mediates the Induction of Chloroplastic Ndh Complex under Photooxidative Stress in Barley Plant Physiology, March 1, 2001; 125(3): 1450 - 1458. [Abstract] [Full Text]
|
|
|
|
|
|
M. Lopez-Serrano, E. M. Del Campo, B. Sabater, and M. Martin Primary transcripts of ndhD of Liliaceae and Aloaceae require editing of the start and 20th codons J. Exp. Bot., January 1, 2001; 52(354): 179 - 180. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. M. Horváth, S. O. Peter, T. Joët, D. Rumeau, L. Cournac, G. V. Horváth, T. A. Kavanagh, C. Schäfer, G. Peltier, and P. Medgyesy Targeted Inactivation of the Plastid ndhB Gene in Tobacco Results in an Enhanced Sensitivity of Photosynthesis to Moderate Stomatal Closure Plant Physiology, August 1, 2000; 123(4): 1337 - 1350. [Abstract] [Full Text]
|
|
|
|
 |
|
 E. M. d. Campo, B. Sabater, and M. Martin Transcripts of the ndhH-D operon of barley plastids: possible role of unedited site III in splicing of the ndhA intron Nucleic Acids Res., March 1, 2000; 28(5): 1092 - 1098. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. Casano, J. M. Zapata, M. Martin, and B. Sabater Chlororespiration and Poising of Cyclic Electron Transport. PLASTOQUINONE AS ELECTRON TRANSPORTER BETWEEN THYLAKOID NADH DEHYDROGENASE AND PEROXIDASE J. Biol. Chem., January 14, 2000; 275(2): 942 - 948. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. José Quiles and J. Cuello Association of Ferredoxin-NADP Oxidoreductase with the Chloroplastic Pyridine Nucleotide Dehydrogenase Complex in Barley Leaves Plant Physiology, May 1, 1998; 117(1): 235 - 244. [Abstract] [Full Text]
|
|
|
|
|
|
A. Haldrup, D. J. Simpson, and H. V. Scheller Down-regulation of the PSI-F Subunit of Photosystem I (PSI) in Arabidopsis thaliana. THE PSI-F SUBUNIT IS ESSENTIAL FOR PHOTOAUTOTROPHIC GROWTH AND CONTRIBUTES TO ANTENNA FUNCTION J. Biol. Chem., September 29, 2000; 275(40): 31211 - 31218. [Abstract] [Full Text] [PDF]
|
|